| 3-苯基乳酸的手性毛细管电泳拆分 |
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| 引用本文： | 李德茂,李从发,刘四新,陈利梅.3-苯基乳酸的手性毛细管电泳拆分[J].色谱,2004,22(3):281-283. |
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| 作者姓名： | 李德茂 李从发 刘四新 陈利梅 |
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| 作者单位： | 华南热带农业大学工学院,海南,儋州,571737 |
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| 摘 要： | 考察了环糊精种类、环糊精浓度、缓冲溶液pH、分离电压、温度等因素对3-苯基乳酸手性分离的影响,并对分离条件进行了优化。结果表明，采用区带毛细管电泳技术，以0.03 mol/L的羟丙基-β-环糊精为手性选择剂,0.1 mol/L的磷酸缓冲溶液(pH 5.5)为电泳缓冲溶液,26 kV的分离电压,在25 ℃下可使3-苯基乳酸对映体达到基线分离,分离度为1.51。
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| 关 键 词： | 3-苯基乳酸 毛细管电泳 羟丙基-β-环糊精 手性分离 |
| 文章编号： | 1000-8713(2004)03-0281-03 |
| 修稿时间： | 2003年8月6日 |
Chiral Separation of 3-Phenyllactic Acid by Capillary Electrophoresis |
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| LI Demao,LI Congfa,LIU Sixin,CHEN Limei.Chiral Separation of 3-Phenyllactic Acid by Capillary Electrophoresis[J].Chinese Journal of Chromatography,2004,22(3):281-283. |
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| Authors: | LI Demao LI Congfa LIU Sixin CHEN Limei |
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| Institution: | College of Technology and Engineering, South China University of Tropical Agriculture, Danzhou 571737, China. |
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| Abstract: | Various factors affecting the chiral resolution of 3-phenyllactic acid by capillary zone electrophoresis such as the type of cyclodextrin, concentration of the chiral selector, buffer pH, voltage, and temperature were studied and optimized. Using 30 mmol/L hydroxypropyl-beta-cyclodextrin as the chiral selector, and 0.1 mol/L phosphate solution (pH 5.5) as the running buffer, the baseline separation of 3-phenyllactic acid enantiomers was achieved with a resolution factor of 1.51. The separation voltage was 26 kV and the temperature was 25 degrees C. |
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| Keywords: | NULL |
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