| 核苷酸锆固定化脂肪酶的制备及其催化性能 |
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| 引用本文： | 陶维红,杨立荣,徐刚,乔元彪,吴坚平.核苷酸锆固定化脂肪酶的制备及其催化性能[J].催化学报,2011,32(4):630-636. |
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| 作者姓名： | 陶维红 杨立荣 徐刚 乔元彪 吴坚平 |
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| 作者单位： | 浙江大学化学工程与生物工程学系; |
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| 基金项目： | 国家自然科学基金重点项目(20936002); 国家高技术研究发展计划(863计划,2010AA101502); 国家重点基础研究发展计划(973计划,2011CB710805); 国家科技支撑计划重点项目(2008BAI63B07)~~ |
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| 摘 要： | 开发了以核苷酸锆纳米颗粒为载体固定化脂肪酶的方法,考察了固定化时间、缓冲液pH值及浓度和酶载量等因素对固定化酶在有机相中催化烯丙醇酮酯交换反应性能的影响.结果表明,一元尿苷酸锆(粒径30～50nm)是较佳的载体,在磷酸缓冲液pH=8.0和浓度为0.03mol/L时,酶与载体质量比为4,固定化6h制得的固定化酶效果最佳....
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| 关 键 词： | 核苷酸锆 纳米颗粒 节杆菌脂肪酶 固定化 酯交换反应 |
Immobilization of Lipase on Zirconium Nucleotide and Its Catalytic Performance |
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| TAO Weihong,YANG Lirong,XU Gang,QIAO Yuanbiao,WU Jianping.Immobilization of Lipase on Zirconium Nucleotide and Its Catalytic Performance[J].Chinese Journal of Catalysis,2011,32(4):630-636. |
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| Authors: | TAO Weihong YANG Lirong XU Gang QIAO Yuanbiao WU Jianping |
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| Institution: | TAO Weihong,YANG Lirong,XU Gang,QIAO Yuanbiao,WU Jianping Department of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310027,Zhejiang,China |
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| Abstract: | The immobilization of Arthrobacter sp.lipase on zirconium nucleotide nanoparticles was investigated.The effect of immobilization time,pH value,buffer concentration,and lipase amount on the catalytic performance of the immobilized lipase in an organic system was studied.The results showed that the enzyme immobilized on zirconium-uridine monophosphate (30-50 nm) had the best catalytic performance for the resolution of 4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-one.The optimal immobilization conditions ... |
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| Keywords: | zirconium nucleotide nanoparticle Arthrobacter sp lipase immobilization transesterification |
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