| 葡萄酒中7种酚酸的色谱电化学分析 |
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| 引用本文： | 曹炜,索志荣,宋纪蓉.葡萄酒中7种酚酸的色谱电化学分析[J].高等学校化学学报,2005,26(8):1424-1427. |
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| 作者姓名： | 曹炜 索志荣 宋纪蓉 |
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| 作者单位： | 西北大学分析科学研究所,陕西省电分析化学重点实验室;西北大学化学工程学院,西安,710069;西北大学分析科学研究所,陕西省电分析化学重点实验室;西北大学化学工程学院,西安,710069 |
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| 基金项目： | 国家自然科学基金;陕西省自然科学基金;陕西省教育厅资助项目;西北大学校科研和教改项目 |
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| 摘 要： | 建立了同时测定葡萄酒中没食子酸、原儿茶酸、丁香酸、p-香豆酸、咖啡酸、绿原酸和阿魏酸等7种生物活性酚酸的反相高效液相色谱电化学分析新方法,并测定了5种国产不同品牌的葡萄酒.采用HypersilODS色谱柱(250mm×4.0mm,5.0μm),流动相为甲醇-4%醋酸,梯度洗脱,流速为0.8mL/min,工作电压为0.7V,柱温为30℃.实验结果表明,电化学法的检出限比紫外法的检出限低4～600倍.
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| 关 键 词： | 高效液相色谱法 电化学检测 葡萄酒 酚酸 |
| 文章编号： | 0251-0790（2005）08-1424-04 |
| 收稿时间： | 09 28 2004 12:00AM |
| 修稿时间： | 2004-09-28 |
Analyses of Seven Kinds of Phenolic Acids in Wine by HPLC with Electrochemical Detection |
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| CAO Wei{,},SUO Zhi-Rong,SONG Ji-Rong,ZHENG Jian-Bin{}.Analyses of Seven Kinds of Phenolic Acids in Wine by HPLC with Electrochemical Detection[J].Chemical Research In Chinese Universities,2005,26(8):1424-1427. |
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| Authors: | CAO Wei{ } SUO Zhi-Rong SONG Ji-Rong ZHENG Jian-Bin{} |
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| Institution: | CAO Wei{1,2},SUO Zhi-Rong1,SONG Ji-Rong2,ZHENG Jian-Bin{1*} |
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| Abstract: | A rapid, sensitive and accurate reverse phase HPLC-ECD method for simultaneous determination of seven kinds of bioactive phenolic acids(gallic acid, protocatechuic acid, syringic acid, p-coumaric acid, caffeic acid, chlorogenic acid, ferulic acid) in five different types of wine was developed. The separation was performed on Hypersil ODS column(250 mm×4.0 mm, 5.0 μm) by gradient elution. The mobile phase consisted of CH-3OH-4%HAc. The flow rate was 0.8 mL/min. The working potential was 0.7 V and column temperature was 30 ℃. The experimental results showed that the seven kinds of phenolic acids were separated satisfactorily. The detection limit of phenolic acids with ECD was 4-600 times greater than those obtained with UV detection. |
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| Keywords: | HPLC Electrochemical detection Wine Phenolic acid |
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