| 高效液相色谱/电化学法测定大鼠血液和脑组织中单胺类物质的含量 |
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| 引用本文： | 谭炳炎,郑琳,冯翔.高效液相色谱/电化学法测定大鼠血液和脑组织中单胺类物质的含量[J].分析测试学报,2006,25(2):90-92,96. |
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| 作者姓名： | 谭炳炎 郑琳 冯翔 |
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| 作者单位： | 1. 中山大学,公共卫生学院中心实验室,广东,广州,510080
2. 中山大学,公共卫生学院营养学系,广东,广州,510080 |
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| 摘 要： | 建立了高效液相色谱／电化学检测法测定大鼠脑组织和血液中单胺递质及其代谢产物的方法。能同时测定去甲肾上腺素（NE）、肾上腺素（E）、3，4-二羟基苯乙酸（DOPAC）、多巴胺（DA）、高香草酸（HAV）、5-羟色胺（5-HT），并能和内标3，4-二羟卞胺（DHBA）分离良好。本方法快速、简便，回收率为92％-105％，线性范围2．8-680ng／mL，检出限为0．05ng（S／N=3）。本方法已应用在服用中药的大鼠下丘脑组织及血液的测定中，数据显示，本法能满足测定要求。
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| 关 键 词： | 高效液相色谱 电化学检测 单胺类神经递质 |
| 文章编号： | 1004-4957（2006）02-0090-03 |
| 收稿时间： | 2005-04-04 |
| 修稿时间： | 2005-04-042005-06-28 |
Determination of Monoamine Transmitters in Rat Blood and Brain Tissues by High Performance Liquid Chromatography |
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| TAN Bing-yan,ZHENG Lin,FENG Xiang.Determination of Monoamine Transmitters in Rat Blood and Brain Tissues by High Performance Liquid Chromatography[J].Journal of Instrumental Analysis,2006,25(2):90-92,96. |
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| Authors: | TAN Bing-yan ZHENG Lin FENG Xiang |
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| Institution: | 1. Central Laboratory, School of Public Health, Sun Yat-sen University, Guangzhou 510089, China; 2. Department of Nutritional Sciences, School of Public Health, Sun Yat-sen University, Guangzhou 510089, China |
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| Abstract: | A method was developed to determine monoamine transmitters and their metabolites in rat blood and brain tissues by high performance liquid chromatography with electrochemical detection.Norepinephrine(NE),epinrphrine(E),3,4-dihydroxyphenylacetic acid(DOPAC),dopamine hydrochloride(DA),homovanillic acid(HVA),and 5-hydroxytryptamine(5-HT) could be determined simultaneously and distinctly separated from internal standard(3,4-dihydroxybenzylamine,DHBA) by this method.The recoveries of these compounds were between 92% and 105% and the linear range was 2.8-680 ng/mL.The detection limit was 0.05 ng(S/N=3).This method was applied to determining the monoamines in hypothalamus tissues of rats upon taking traditional Chinese medicine,and satisfactory results were achieved. |
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| Keywords: | High performance liquid chromatography Electrochemical detection Monoamines |
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