Dendritic Silver/Silicon Dioxide Nanocomposite Modified Electrodes for Electrochemical Sensing of Hydrogen Peroxide |
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| Authors: | Peixi Yuan Ying Zhuo Yaqin Chai Huangxian Ju |
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| Institution: | 1. School of Chemistry and Chemical Engineering, Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Nanjing University, Nanjing 210093, P.?R. China;2. Key Laboratory of Analytical Chemistry (Chongqing), College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P.?R. China |
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| Abstract: | A novel biosensor for hydrogen peroxide was prepared by immobilizing horseradish peroxidase (HPR) on newly synthesized dendritic silver/silicon dioxide nanocomposites, which were coated on a glassy carbon electrode. The modified electrode was characterized with XPS, SEM, and electrochemical methods. This biosensor showed a very fast amperometric response to hydrogen peroxide with a linear range from 0.7 to 120 μM, a limit of detection of 0.05 μM and a sensitivity of 1.02 mA mM?1 cm?2. The Michaelis‐Menten constant of the immobilized HRP was estimated to be 0.21 mM, indicating a high affinity of the HRP to H2O2 without loss of enzymatic activity. The preparation of the proposed biosensor was convenient, and it showed high sensitivity and good stability. |
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| Keywords: | Silicon dioxide nanoparticles Nanocomposites Deposition Silver Amperometric biosensor Hydrogen peroxide |
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