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Fiber Optic Spectrophotometer for Noninvasive Transmission and Diffuse Reflection Studies
Authors:N Kollias  A Baqer  K Razi Naqvi
Institution:1. Department of Dermatology , Al-Sabah Hospital , Kuwait;2. Physics Department , Kuwait University , Kuwait;3. Department of Dermatology , Al-Sabah Hospital , Kuwait;4. Physics Department , Kuwait University , Kuwait
Abstract:We describe the construction of a simple but versatile single-beam spectrophotometer that uses no sample holder at all; one arm of a bifurcated fiber optic bundle illuminates the specimen, while the other receives the remitted light. Though designed with an eye to recording the diffuse reflection spectra of scattering-and-absorbing substances, it can also be used for conventional studies (ie transmission spectroscopy) by merely placing a plane mirror behind the cuvette. The performance of the instrument is evaluated and representative spectra of several powders, solutions, and biomaterials are presented.
Keywords:Fiber optics  Diffuse reflection spectroscopy  CdS  TiO2  Ho2O3  Nd2O3  oxyhemoglobin
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