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Electrospun Nanofibers Modified with Phospholipid Moieties for Enzyme Immobilization
Authors:Xiao‐Jun Huang  Zhi‐Kang Xu  Ling‐Shu Wan  Christophe Innocent  Patrick Seta
Abstract:Summary: PANCMPCs containing phospholipid side moieties were electrospun into nanofibers with a mean diameter of 90 nm. Field emission SEM was used to characterize the morphologies of the nanofibers. These phospholipid‐modified nanofibers were explored as supports for enzyme immobilization due to the characteristics of excellent biocompatibility, high surface/volume ratio, and porosity, which were beneficial to the catalytic efficiency and activity of immobilized enzymes. Lipase from Candida rugosa was immobilized on these nanofibers by adsorption. Preliminary results indicated that the properties of the immobilized lipase on these phospholipid‐modified nanofibers were greatly promising.
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Schematic representation of the structure and electrostatic properties of phospholipid‐modified nanofibers.

Keywords:biomimetic polymer  electrospinning  enzyme immobilization  lipase  nanofibers  phospholipids
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