| OAP-H2O2-HRP酶促反应产物与DNA相互作用的光谱及电化学研究 |
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| 引用本文： | 张书圣,牛淑妍,马立波,焦奎.OAP-H2O2-HRP酶促反应产物与DNA相互作用的光谱及电化学研究[J].高等学校化学学报,2004,25(4):630-632. |
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| 作者姓名： | 张书圣 牛淑妍 马立波 焦奎 |
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| 作者单位： | 1. 青岛科技大学化学与分子工程学院,青岛,266042
2. 青岛科技大学化学与分子工程学院,青岛,266042;中国海洋大学化学与化工学院,青岛,266003 |
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| 基金项目： | 国家自然科学基金(批准号: 20275020)和山东省自然科学基金(批准号: Z2002B02)资助. |
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| 摘 要： | 脱氧核糖核酸(DNA)是生物的基本遗传物质，对其研究是生命科学研究领域中极其重要的内容，其中有关DNA靶向分子与DNA之间相互作用的研究一直是一个比较活跃的领域，继Bard等用电化学方法对溶液中的电活性物质与DNA相互作用进行研究之后，又有许多相关的研究成果相继报道。3-氨基酚噁嗪(AP，即寻霉素A)与放线菌素D的生成有关，放线菌素D在伴随DNA指导RNA的合成中起作用，AP被用作放线菌素D的行为模型，对放线菌素D还原成1个N-10中心阴离子提供信息。
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| 关 键 词： | 3-氨基酚嗪 DNA 循环伏安法 紫外-可见光谱 嵌插作用 |
| 文章编号： | 0251-0790(2004)04-0630-03 |
| 收稿时间： | 2003-06-11 |
Spectroscopic and Electrochemical Studies on the Interaction Between DNA and the Product of the Enzyme-catalyzed Reaction of OAP-H2O2-HRP |
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| ZHANG Shu-Sheng ,NIU Shu-Yan ,MA Li-Bo ,JIAO Kui.Spectroscopic and Electrochemical Studies on the Interaction Between DNA and the Product of the Enzyme-catalyzed Reaction of OAP-H2O2-HRP[J].Chemical Research In Chinese Universities,2004,25(4):630-632. |
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| Authors: | ZHANG Shu-Sheng NIU Shu-Yan MA Li-Bo JIAO Kui |
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| Institution: | ZHANG Shu-Sheng 1,NIU Shu-Yan 1,2,MA Li-Bo 1,JIAO Kui 1* |
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| Abstract: | The pure product of 2-aminophenoxazine-3-one was prepared by enzyme-catalyzed reaction and analyzed. The electrochemical behaviour of 2-aminophenoxazine-3-one on a glass carbon electrode was investigated. The interaction between DNA and 2-aminophenoxazine-3-one was investigated by cyclic voltammetry and UV-Vis spectroscopy. The peak potential of 2-aminophenoxazine-3-one in pH 6.8 phosphate buffer solution in the presence of DNA shifts positively, indicating that the bind of 2-aminophenoxazine-3-one with DNA is intercalation action. The influence of reacting time was also studied. The binding ratio of the complex of DNA and 2-aminophenoxazine-3-one at room temperature is calculated to be 1∶1, and the binding constant is to be 6.60×104L/mol. |
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| Keywords: | 2-Aminophenoxazine-3-one DNA Cyclic voltammetry UV-Vis spectroscopy Intercalation |
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