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Chiral separation of phenylalanine in ultrafiltration through DNA-immobilized chitosan membranes |
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| Authors: | Yuki Matsuoka Naoki Kanda Young Moo Lee Akon Higuchi |
| Institution: | aPostgraduate Course of Applied Chemistry, Seikei University, Tokyo 180-8633, Japan bDepartment of Materials and Life Science, Seikei University, Tokyo 180-8633, Japan cNational Research Laboratory for Membranes, School of Chemical Engineering, Hanyang University, Seoul 133-791, South Korea |
| Abstract: | Ultrafiltration experiments for the chiral separation of racemic phenylalanine were performed with DNA-immobilized chitosan membranes having various pore sizes. Atomic analysis on the membranes showed that the chitosan membranes covalently bound six times more DNA than the cellulose membranes used in our previous study A. Higuchi, Y. Higuchi, K. Furuta, B.O. Yoon, M. Hara, S. Maniwa, M. Saitoh, K. Sanui, Chiral separation of phenylalanine by ultrafiltration through immobilized DNA membranes, J. Membr. Sci. 221 (2003) 207–218]. d-Phenylalanine preferentially permeated through DNA-immobilized chitosan membranes with a pore size <6.4 nm molecular weight cut-off (MWCO) <67,000]. The binding affinity of a specific enantiomer due to the pore size of the DNA-immobilized membranes regulated the preferential permeation of the enantiomer through the membranes. l-Phenylalanine was adsorbed on the DNA-immobilized chitosan membranes with a pore size <6.4 nm (MWCO < 67,000), while there was little difference between the adsorption of d-phenylalanine and l-phenylalanine on the membranes with a pore size >6.4 nm (MWCO > 67,000). The DNA-immobilized chitosan membranes were categorized as channel type membranes. |
| Keywords: | Chiral separation Membrane DNA Chitosan Ultrafiltration |
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