| Synthesis of affinity column of phosphatidylinositol-3,4-diphosphate |
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| 作者姓名： | OZAKI Shoichiro WATANABE Yutaka OGASAWARA Tomio |
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| 作者单位： | Department of Chemistry，Shandong University，Jinan，Shandong 250100，China，Department of Applied Chemistry，Faculty of Engineering，Ehime University，Matsuyama，790，Japan，Department of Applied Chemistry，Faculty of Engineering，Ehime University，Matsuyama，790，Japan |
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| 摘 要： | Phosphatidylinositol polyphosphates (PIPx) are related with tyrosine kinase activation, cell proliferation and carcinogenesis. In order to investigate the action mechanism of PIPx, it is desirable to synthesize affinity column of PI-3,4-P2, which is expected to be able to isolate the binding proteins of PI-3,4-P2. Thyramine reacted with CH-Sepharose 4B giving column 13. The p-amino group of 3'-(1',2'-distearoyl-glyceryl)-1-(2-p-aminobenzyl)-3,4-di-O-phosphoryl-myo-inosityl phosphate (12) was diazotized, then diazo-coupled with column 13 to give PI-3,4-P2 affinity column 14. This PI-3,4-P2 affinity column is an effective tool to pick up binding proteins of PI-3,4-P2.
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Synthesis of affinity column of phosphatidylinositol-3,4-diphosphate |
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| OZAKI Shoichiro,WATANABE Yutaka,OGASAWARA Tomio.Synthesis of affinity column of phosphatidylinositol-3,4-diphosphate[J].Chinese Journal of Chemistry,1997,15(6):556-561. |
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| Authors: | OZAKI Shoichiro KONG Xiang-Zheng WATANABE YutakaOGASAWARA Tomio |
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| Institution: | OZAKI,Shoichiro KONG,Xiang-Zheng WATANABE,YutakaOGASAWARA,Tomio Department of Chemistry,Shandong University,Jinan,Shandong 250100,China Department of Applied Chemistry,Faculty of Engineering,Ehime University,Matsuyama,790,Japan |
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| Abstract: | Phosphatidylinositol polyphosphates (PIPx) are related with tyrosine kinase activation, cell proliferation and carcinogenesis. In order to investigate the action mechanism of PIPx, it is desirable to synthesize affinity column of PI-3,4-P2, which is expected to be able to isolate the binding proteins of PI-3,4-P2. Thyramine reacted with CH-Sepharose 4B giving column 13. The p-amino group of 3'-(1',2'-distearoyl-glyceryl)-1-(2-p-aminobenzyl)-3,4-di-O-phosphoryl-myo-inosityl phosphate (12) was diazotized, then diazo-coupled with column 13 to give PI-3,4-P2 affinity column 14. This PI-3,4-P2 affinity column is an effective tool to pick up binding proteins of PI-3,4-P2. |
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| Keywords: | Phosphatydylinositol-3 4-diphosphate binding protein inositol PI-3 4-P2-affinity column |
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