A label-free and signal-on supersandwich fluorescent platform for Hg sensing |
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Authors: | Tao Yuan Lianzhe Hu Zhongyuan Liu Wenjing Qi Shuyun Zhu Aziz-ur-Rehman Guobao Xu |
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Institution: | 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China;2. University of the Chinese Academy of Sciences, PR China;3. Department of Chemistry, Baghdad-ul-Jadeed Campus, The Islamia University of Bahawalpur, Bahawalpur, Pakistan |
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Abstract: | A label-free supersandwich fluorescent assay was demonstrated for the first time by taking Hg2+ as a detection candidate. The principle of the proposed supersandwich fluorescent platform is based on the formation of supersandwich structure by T-Hg2+-T coordination and the fluorescence enhancement of the intercalated Genefinder (GF) in double strand DNA (dsDNA). Such supersandwich fluorescent DNA sensor exhibits a linear range of 10–300 nM for the detection of Hg2+, with a detection limit of 2.5 nM on the basis of the 3σ/slope (σ represents the standard deviation of the blank samples), which is well below the permit of the U.S. Environmental Protection Agency (<10 nM). The detection can be fulfilled in less than 10 min. The proposed mix-and-detect fluorescent platform exhibits excellent sensitivity, selectivity, and convenient manipulation. The assay was successfully used to detect Hg2+ in the lake water samples, which suggested its potential in practical samples. |
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Keywords: | Fluorescence Label-free Supersandwich Signal-on Hg2+ |
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