| 以1,4-二甲基咔唑-9-丙酸为荧光衍生化试剂高效液相色谱法测定醇 |
| |
| 引用本文： | 劳文剑,宋翠华,尤进茂,欧庆瑜.以1,4-二甲基咔唑-9-丙酸为荧光衍生化试剂高效液相色谱法测定醇[J].化学研究,2013(1):75-78,81. |
| |
| 作者姓名： | 劳文剑 宋翠华 尤进茂 欧庆瑜 |
| |
| 作者单位： | 中国科学院兰州化学物理研究所;曲阜师范大学化学与化工学院 |
| |
| 摘 要： | 1,4-二甲基咔唑-9-丙酸与间苯二磺酰氯反应生成混合酸酐,该混合酸酐能够衍生化18个碳以下的直链脂肪伯醇、6个碳以下的仲醇,以及叔丁醇和胆固醇;将荧光衍生物用反相C18液相色谱柱进行分离,在室温下10min完成衍生化反应,衍生物24h内基本稳定.基于此,建立了血清中醇的高效液相色谱测定方法.
|
| 关 键 词： | 血清 醇 1 4-二甲基咔唑-9-丙酸 荧光衍生 高效液相色谱 测定 |
Determination of alcohols by HPLC with 1,4-dimethylcarbazole-9-yl-propionic acid as fluorescence derivative reagent |
| |
| LAO Wen-jian,SONG Cui-hua,YOU Jin-mao,OU Qing-yu.Determination of alcohols by HPLC with 1,4-dimethylcarbazole-9-yl-propionic acid as fluorescence derivative reagent[J].Chemical Research,2013(1):75-78,81. |
| |
| Authors: | LAO Wen-jian SONG Cui-hua YOU Jin-mao OU Qing-yu |
| |
| Institution: | 1(1.Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,Gansu,China; 2.School of Chemistry and Chemical Engineering,Qufu Normal University,Qufu 273165,Shandong,China) |
| |
| Abstract: | Pre-column derivative reagent 1,4-dimethyl-carbazole-9-yl-propionic acid was allowed to couple with benzenedisulfonyl dichloride affording the mixed acidic anhydride. Resultant mixed acidic anhydride was used to derivate straight-chain aliphatic primary alcohols with less than 18 carbons and secondary alcohols with less than 6 carbons, as well as tert-butanol and cholesterol. As-obtained fluorescence derivatives were separated on reversed-phase C18 high- performance liquid chromatography （HPLC） column, with which the derivatization was fin- ished in 10 min at room temperature and relevant derivatives were stable within 24 h. This makes it feasible to establish a HPLC method to determine alcohols in human serum with 1,4- dimethyl-carbazole-9-yl-propionic acid as the fluorescence derivative reagent. |
| |
| Keywords: | serum alcohol 1 4-dimethyl-carbazole-9-yl-propionic acid fluorescence derivatiza-tion HPLC determination |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
