| 反相HPLC测定食物中的有机酸 |
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| 引用本文： | 李连朝, 李天荣, 汪沛洪.反相HPLC测定食物中的有机酸[J].分析测试学报,1989(3). |
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| 作者姓名： | 李连朝 李天荣 汪沛洪 |
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| 作者单位： | 陕西杨陵西北农业大学中心实验室
(李连朝，李天荣)，陕西杨陵西北农业大学中心实验室(汪沛洪) |
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| 摘 要： | 本文研究了反相HPLC同时测定食物中草酸、酒石酸、苹果酸、乳酸、乙酸、柠檬酸和琥珀酸的条件,提出了用C_(18)固定相、2.5%NH_4H_2PO_4(pH2.50)溶液的流动相和在200nm下紫外检测这七种有机酸的测定方法。该法简便快速、具有较高的准确度和精密度。另外,还对有机酸的分离机理作了探讨。对十种食物中的有机酸进行了定量测定。
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| 关 键 词： | 有机酸 食物 反相HPLC |
Determination of Organic Acids in Foodstuffs by Reversed-phase HPLC |
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| Li Lianchao,Li Tianrong,Wang Peihong Central Laboratory of Northwestern Agricultural University,Yangling Shaanxi Province.Determination of Organic Acids in Foodstuffs by Reversed-phase HPLC[J].Journal of Instrumental Analysis,1989(3). |
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| Authors: | Li Lianchao Li Tianrong Wang Peihong Central Laboratory of Northwestern Agricultural University Yangling Shaanxi Province |
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| Abstract: | The conditions of simultaneous determination of oxalic, tartaric, malic, lactic, acetic, citricand succinic acid in foodstuffs by reversed-phase HPLC have been studied. The method on amicrobondapak c_(18) Column, with 2.5% NH_4H_2PO_4 (pH 2.50) as eluent and UV detection at200nm, was established. This assay features straightforward sample preparation, higher accuracyand precision. Moreover, the separation mechanisms of these acids was also discussed, and theacids in 10 foodstuff samples were determined with satisfactory results. |
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| Keywords: | Organie acid Foodstuff IReversed-phase HPLC |
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