一种乙肝病毒表面抗原和其单克隆抗体相互作用的SERS研究

利用银纳米粒子的表面增强拉曼散射（SERS）效应，研究了乙肝病毒表面抗原 （HBsAg)和其鼠源单克隆抗体（单抗，Ab-HBsAg)的相互作用。SERS光谱结果表明 ，Ab-HBsAg分子主要通过位于非抗原结合部位的去质子化羧基（COO）^-实现与银 纳米粒子的结合。HBsAg与Ab-HBsAg相互作用形成免疫复合物后，HBsAg分子上的色 氨酸（Trp)残基特征振动完全消失，表明Trp残基位于HBsAg抗原分子的活性区，是 HBsAg与Ab-HBsAg相互作用的重要位点。

Surface-Enhanced Raman Spectroscopic Study on the Interaction of Hepatitis B Virus Surface Antigen and Its Monoclonal Antibody

Surface-enhanced Raman scattering (SERS) has been employed to study the interaction of hepatitis B virus surface antigen (HBsAg) with mouse monoclonal antibody against HBsAg (Ab-HBsAg). SERS is used to obtain spectra of HBsAg, Ab-HBsAg and their immune complex adsorbed on colloidal silver. It is found that Ab-HBsAg is bound to silver colloids through the carboxyl groups, which are not on the antigen binding site of the Ab-HBsAg. The vibrations from tryptophan residues of the HBsAg disappear after formation of the immune complex. It is assumed that the tryptophan residues are among the antigen active sites of the HBsAg and play an important role in the interaction between Ab-HBsAg and HBsAg.