| 氨基酸在薄层原位的傅里叶变换表面增强拉曼光谱 |
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| 引用本文： | 汪瑗,张煦.氨基酸在薄层原位的傅里叶变换表面增强拉曼光谱[J].分析化学,1998,26(9):1047-1051. |
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| 作者姓名： | 汪瑗 张煦 |
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| 作者单位： | [1]首都师范大学化学系 [2]北京大学化学系 |
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| 基金项目： | 北京中关村联合测试中心分析测试基金资助课题 |
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| 摘 要： | 应用表面增强技术将薄层色谱与红外傅里叶变换拉曼光谱联用，在薄层色谱原位获得两种氨基酸光谱。研究表明，在薄层原位微量样品的ＮＩＲ－ＦＴ－ＳＥＲＳ光谱与纯固体样品ＦＴ－Ｒａｍａｎ光谱的主要特征谱带频率基本一致，在银微粒作用下，色氨酸分子中吲哚环环伸缩振动获最大增强，组氨酸分子中Ｃ＝Ｎ双键的伸缩振动是明显增强，对８μｇ样品就能在薄层色谱原位较可靠地反映了分子的结构信息。
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| 关 键 词： | 色氨酸 组氨酸 薄层色谱 FT SERS 联用 测定 |
In Situ Thin Layer Chromatography -Fourier Transform -Surface Enhanced Raman Scattering Spectroscopy of Amino Acid |
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| Wang Yuan,Yu Bingzheng,Wang Ting.In Situ Thin Layer Chromatography -Fourier Transform -Surface Enhanced Raman Scattering Spectroscopy of Amino Acid[J].Chinese Journal of Analytical Chemistry,1998,26(9):1047-1051. |
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| Authors: | Wang Yuan Yu Bingzheng Wang Ting |
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| Abstract: | Near-infrared-Fourier transform surface-enhanced Raman scattering spectroscopy of two amino acids, tryptophan and histidine, in thin layer chromatography (TLC) is presented. In situ identification of TLC, the spectral featare of 8ug amino acid spots obtained was same as that of solid sample. In addition, the sensitivitiy was improved greatly by using activated silver colloid in thin layer chromatographiy. |
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| Keywords: | Tryptophan histidine thin layer chromatography near-infrared-Fourier transform spectrometry surface- enhanced - Raman spectrometry |
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