| HPLC法同时测定尿样中芳香族化合物的6种代谢产物 |
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| 引用本文： | 徐烨,张春妮,曹轶男,徐梵姝,王乃芝.HPLC法同时测定尿样中芳香族化合物的6种代谢产物[J].分析测试学报,2011,30(9):1028-1032. |
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| 作者姓名： | 徐烨 张春妮 曹轶男 徐梵姝 王乃芝 |
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| 作者单位： | 东北大学理学院,辽宁沈阳,110004 |
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| 摘 要： | 建立了同时测定尿样中反,反-粘康酸、马尿酸、苯乙醇酸、苯乙醛酸、对氨基酚和对硝基酚的高效液相色谱法(HPLC).优化了色谱分离及检测条件,并采用时间程序波长检测.确定了最佳样品预处理条件:以5 mL二氯甲烷-异丙醇(7∶3)为萃取溶剂；样品用量5 mL；NaCI加入量0.75 g；萃取时间2 min,分别在酸性和中性条...
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| 关 键 词： | 高效液相色谱法 尿样 代谢产物 液-液萃取 |
Simultaneous Determination of Six Urinary Metabolites of Aromatic Compounds by HPLC |
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| XU Ye,ZHANG Chun-ni,CAO Yi-nan,XU Fan-shu,WANG Nai-zhi.Simultaneous Determination of Six Urinary Metabolites of Aromatic Compounds by HPLC[J].Journal of Instrumental Analysis,2011,30(9):1028-1032. |
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| Authors: | XU Ye ZHANG Chun-ni CAO Yi-nan XU Fan-shu WANG Nai-zhi |
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| Institution: | XU Ye,ZHANG Chun-ni,CAO Yi-nan,XU Fan-shu,WANG Nai-zhi(College of Science,Northeastern University,Shenyang 110004,China) |
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| Abstract: | A high performance liquid chromatographic(HPLC) method was established for the simultaneous determination of trans,trans-muconic acid(tt-MA),hippuric acid(HA),mandelic acid(MA),phenylglyoxy acid(PGA),p-aminophenol(PAP)and p-nitrophenol(PNP)in urine.The chromatographic separation and the detection conditions were optimized,and a programmed gradient which the wavelength was changed with the time was applied for the detection.The optimal pretreatment conditions for the sample were as follows:extraction solvent... |
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| Keywords: | HPLC urine metabolite liquid-liquid extraction |
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