铽-依诺沙星-SDS敏化荧光猝灭法检测雷公藤红素

在pH 9.3的氨-氯化铵缓冲溶液中,铽(Ⅲ)能与依诺沙星、十二烷基硫酸钠(SDS)形成荧光配合物(λex=330 nm、λem=545 nm),SDS的存在能增强配合物的荧光强度。研究发现,在该反应体系中加入适量雷公藤红素溶液后,铽(Ⅲ)与依诺沙星络合物的激发、发射峰位置不变,但其荧光强度呈规律性下降。据此,建立了简单、快速、灵敏地测定雷公藤红素的荧光分析方法。雷公藤红素的浓度在5.2×10-6～8.4×10-5 mol/L范围内呈良好线性关系,方法的检出限为4.1×10-8 mol/L。

Determination of Celastrol by Lanthanide Sensitized Fluorescence Quenching on Terbium- Enoxacin-SDS

In NH3-NH4Cl buffer solution(pH 9.3),terbium(Ⅲ) reacted with enoxacin(ENX) to form fluorescence coordinate compound with the maximum excitation wavelength of 330 nm and the maximum emission wavelength of 545 nm.The presence of SDS obviously strengthened the fluorescence intensity,but the fluorescence intensity of the complex was diminished by continuously adding celastrol,while the locations of the excitation and emission wavelengths were kept the same.Based on this,a new fluorescence method was proposed for the determination of celastrol.The method is simple,rapid and sensitive.The linear range of celastrol is 5.2×10-6-8.4×10-5 mol/L and the detection limit is 4.1×10-8 mol/L.