| 巯基乙酸自组装膜DNA电化学传感器对转基因NOS的定量检测 |
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| 引用本文： | 孙伟,尚智美,杨茂霞,焦奎.巯基乙酸自组装膜DNA电化学传感器对转基因NOS的定量检测[J].高等学校化学学报,2006,27(10):1859-1861. |
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| 作者姓名： | 孙伟 尚智美 杨茂霞 焦奎 |
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| 作者单位： | 青岛科技大学化学与分子工程学院,青岛,266042 |
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| 摘 要： | 以转基因植物中常用的根癌农杆菌终止子(NOS)为检测对象, 将巯基乙酸自组装于金电极表面形成巯基乙酸自组装单分子膜, 再利用乙基-(3-二甲基氨丙基)碳二亚胺盐酸盐(EDC)和N-羟基琥珀酰亚胺(NHS)的活化作用将NOS探针ssDNA序列固定于金电极表面形成NOS电化学生物传感器, 以亚甲基蓝(MB)为杂交指示剂, 对NOS靶基因相关序列进行了定量检测.
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| 关 键 词： | 电化学基因传感器 巯基乙酸 NOS基因 亚甲基蓝 自组装膜电极 |
| 文章编号： | 0251-0790（2006）10-1859-03 |
| 收稿时间： | 01 9 2006 12:00AM |
| 修稿时间： | 2006-01-09 |
Mercaptoacetic Acid Self-assembled DNA Electrochemical Biosensor for Detection of NOS Gene Sequence |
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| SUN Wei,SHANG Zhi-Mei,YANG Mao-Xia,JIAO Kui.Mercaptoacetic Acid Self-assembled DNA Electrochemical Biosensor for Detection of NOS Gene Sequence[J].Chemical Research In Chinese Universities,2006,27(10):1859-1861. |
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| Authors: | SUN Wei SHANG Zhi-Mei YANG Mao-Xia JIAO Kui |
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| Institution: | College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China |
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| Abstract: | A NOS gene electrochemical biosensor was prepared by covalently mmobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride(EDC) and N-hydroxysuccinimide(NHS). By using methylene blue as the electrochemical indicator, the increase of cathodic peak current was in linear with the concentration of complementary ssDNA in the concentration range of 5.0×10-8—1.0×10-4 mol/L with the linear regression equation as ΔIpc=1.10lgc+8.25(n=8, γ=0.998 5) and the detection limit as 3.60×10-8 mol/L(3σ). |
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| Keywords: | Electrochemical DNA biosensor Mercaptoacetic acid NOS gene Methylene blue Self-assembled film electrode |
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