| 代谢组学GC-MS分析方法中样品衍生化技术的新进展 |
| |
| 引用本文： | 熊喜悦,盛小奇,王华,王鷁超.代谢组学GC-MS分析方法中样品衍生化技术的新进展[J].化学通报,2015,78(7):602-607. |
| |
| 作者姓名： | 熊喜悦 盛小奇 王华 王鷁超 |
| |
| 作者单位： | 湖南省小儿遗传代谢性疾病防治临床医疗技术研究中心湖南省妇幼保健院 长沙410008 |
| |
| 基金项目： | 湖南省科技厅专项2013XC83-11和2014SK4044 |
| |
| 摘 要： | 代谢组学是通过考察生物体系受刺激或扰动前后(如某个特定的基因变异或环境变化后)代谢产物的动态变化,研究生物体系代谢网络的一种技术,其研究对象主要是内源性小分子物质.这些物质大部分极性强,难挥发,在气相色谱-质谱分析前,需要进行适当的化学衍生处理.近几年来化学衍生技术发展迅速,本文主要从衍生试剂种类、衍生条件和衍生效果(包括衍生效率、重复性和产物稳定性等)出发,综述了已报道的衍生方法的特点,并展望了衍生技术的发展前景.
|
| 关 键 词： | 代谢组学 化学衍生 肟化-硅烷化 烷基化 衍生效率 |
| 收稿时间： | 2014/12/1 0:00:00 |
| 修稿时间： | 2015/1/20 0:00:00 |
Development of chemical derivatization in metabolites analysis by GC-MS |
| |
| Xiyue Xiong,Xiaoqi Sheng,Hua Wang and Yichao Wang.Development of chemical derivatization in metabolites analysis by GC-MS[J].Chemistry,2015,78(7):602-607. |
| |
| Authors: | Xiyue Xiong Xiaoqi Sheng Hua Wang and Yichao Wang |
| |
| Abstract: | Metabolomics involves the holistic analysis of the changes in the complete set of metabolites (small organic compounds, MW < 1000) in the cell, body fluids, or tissue. However, many metabolites contain polar functional groups that are thermally labile at the temperature required for their separation by GC-MS or are not volatile at all. Therefore, suitable derivatization of these compounds prior to GC-MS analysis is necessary. Herein, based on derivatization reagents, reaction conditions, derivatization efficiencies and stability of the derivatives, derivatization methods are comprehensively summarized and future development prospects were presented. |
| |
| Keywords: | metabolomics chemical derivatization tandem oximation-silylation alkylation derivatization efficiency |
|
| 点击此处可从《化学通报》浏览原始摘要信息 |
| 点击此处可从《化学通报》下载免费的PDF全文 |
|
