| 裂殖壶菌对7种抗生素的敏感性 |
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| 引用本文： | 李清,臧晓南,张学成,许永,鹿宁.裂殖壶菌对7种抗生素的敏感性[J].武汉大学学报(理学版),2012,58(3):275-280. |
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| 作者姓名： | 李清 臧晓南 张学成 许永 鹿宁 |
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| 作者单位： | 中国海洋大学海洋生命学院,山东青岛,266003 |
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| 基金项目： | 国家高技术研究发展计划(863)，国家科技支撑计划 |
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| 摘 要： | 实验室条件下研究了Zeocin、两性霉素B(Amphotericin B)、G418、氯霉素(Cm)、氨苄青霉素(Amp)、卡那霉素(Km)、链霉素(Str)7种基因工程常用抗生素对裂殖壶菌(Schizochytrium limacinum)生长的影响。结果表明:S.limacinum对Zeocin最为敏感,在2.5～4mg.L-1 Zeocin中成活率降至2.10%～2.79%(P<0.01);对两性霉素B、G418、Cm较敏感,在14～20mg.L-1两性霉素B中的成活率降至5%以下(P<0.01),在140mg.L-1G418中的成活率为2.08%(P<0.01),在25.5～68mg.L-1 Cm中成活率为13%左右(P<0.05);对Amp,Km,Str不敏感,50～300mg.L-1的Amp,Km,Str不能抑制其生长,成活率仍在80%以上(P>0.05).
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| 关 键 词： | 裂殖壶菌 抗生素 敏感性 成活率 |
Sensitivities of Schizochytrium limacinum to Seven Antibiotics |
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| LI Qing,ZANG Xiaonan,ZHANG Xuecheng,XU Yong,LU Ning.Sensitivities of Schizochytrium limacinum to Seven Antibiotics[J].JOurnal of Wuhan University:Natural Science Edition,2012,58(3):275-280. |
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| Authors: | LI Qing ZANG Xiaonan ZHANG Xuecheng XU Yong LU Ning |
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| Institution: | (College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China) |
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| Abstract: | The sensitivities of Schizochytrium limacinum to seven antibiotics were reported here,including Zeocin,Amphotericin B,G418,chloramphenicol(Cm),ampicillin(Amp),kanamycin(Km) and streptomycin(Str).The results show that S.limacinum has the most sensitivity to Zeocin and then to Amphotericin B,G418 and Cm in sequence.The survival rate of S.limacinum was only 2.10%-2.79%(P<0.01) while Zeocin’s concentration was at 2.5-4.0 mg·L-1.The survival rate of S.limacinum cultured with Amphotericin B,G418 and Cm over 5%(P<0.01),2.08%(P<0.01) and 13%(P<0.05) respectively under the higher concentration of each antibiotics which was 14-20 mg·L-1,140 mg·L-1 and 25.5-68 mg·L-1.The results indicate that S.limacinum is not sensitive to Amp,Km and Str since high content of these antibiotics(50-300 mg·L-1) can not inhibit the growth of S.limacinum and its survival rate was still over 80%(P>0.05). |
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| Keywords: | Schizochytrium antibiotics sensitivity survival rate |
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