毛细管电泳手性选择剂去硫酸基硫酸软骨素C的研究开发及新药西尼地平对映体的拆分

研究开发了一种新型的毛细管电泳多糖手性选择剂去硫酸基硫酸软骨素C，并用于二氢吡啶类药物对映体的分离。建立了新药西尼地平对映体的拆分方法，同时考察了背景电解质pH值、手性添加剂浓度、工作电压等因素对手性分离的影响。优化的背景电解质pH值 为2.50、手性添加剂的质量浓度为30 g/L，工作电压为10 kV。以去硫酸基硫酸软骨素C为毛细管电泳手性选择剂拆分新药西尼地平对映体，操作简单方便，西尼地平两对映体得到了基线分离，分离度达2.01。

Development of Desulfated Chondroitin Sulfate C as a Novel Chiral Selector in Capillary Electrophoresis and Enantioseparation of Cilnidipine

Desulfated chondroitin sulfate C, a synthetic polysaccharide, was successfully developed as a novel chiral selector in capillary electrophoresis. It was first applied to the enantioseparation of the drugs of dihydropyridine class. The racemic mixture of cilnidipine, a new calcium channel antagonist for the treatment of hypertension, was resolved for the first time. The effects of buffer pH, concentration of the chiral additive and applied voltage on the chiral separation were investigated. The optimum conditions were pH 2.50, 30 (g/L) for additive concentration and 10 kV for applied voltage. Under the optimized conditions the enantiomers of cilnidipine were completely separated with a resolution factor of 2.01. The method is simple and rapid with high separation efficiency.