双柱气相色谱法测定明胶空心胶囊中环氧乙烷的残留量

采用弱极性、中极性的毛细管柱互补分离技术对明胶空心胶囊中的环氧乙烷进行定性、定量分析。样品用水提取,顶空进样,经DB–624或PLOT/Q色谱柱分离,氢火焰离子化检测器检测,外标法定量。结果表明,在0.1~1.6 mg/L内线性关系良好(r≥0.998),检出限为0.15 mg/kg,加标回收率为98.2%~105.1%,测定结果的相对标准偏差为3.6%~6.2%(n=6)。该方法操作简便、灵敏度高,能满足明胶空心胶囊中环氧乙烷的检测要求。

Determination of Ethylene Oxide in Gelatin Hollow Capsules by Dual Capillary Column Gas Chromatography

A method was developed for the determination of ethylene oxide in gelatin hollow capsules by using complementary separation on a dual capillary column system with low and intermediate polarities. The ethylene oxide in gelatin hollow capsules was extracted by water. The headspace vapor of the final extract was injected into DB-624 or PLOT/Q gas chromatography column,and detected by hydrogen flame ionization detector (FID). The target compound was quantified by external standard. A good linearity was obtaned between 0.1 mg/L and 1.6 mg/L with the correlation coefficientr not less than0.998,and the dection limit was 0.15 mg/kg. The recoveries were 98.2%-105.1% with the relative standard deviation of 3.6%-6.2%(n=6). The method is simple,has high sensitivity,which can meet the detection requirements of ethylene oxide in gelatin hollow capsules.