带有可更换半自准工作电极的集成化毛细管电泳安培检测芯片

自Woolley等首次报道集成于玻璃芯片上的微型毛细管电泳-安培检测(Chip-based capillary electrophoresis with amperometric detection，CE-AD)系统以来，CE-AD以其高效、高速、高灵敏度以及易微型化集成化等特点引起研究者的关注．在芯片上实现柱端安培检测可用直接制作在芯片上的喷(镀)膜工作电极，或采用外置的壁喷式电极。前者集成化程度高，后者的工作电极可以更换，大大提高了芯片的重复利用率。

An Integrated Capillary Electrophoresis-Amperometric Detection Chip with Replaceable and Quasi-self-aligned Working Electrode

A glass capillary electrophoresis microchip with integrated amperometric detection cell equipped by replaceable micro-disk working electrode is described. The detection cell was fabricated as a buffer reservoir on the microchip. A micro-electrode-guiding hole, which was in alignment with the exit of the separation channel, was drilled into the bottom edge of the chip. When a micro-disk copper working electrode, the outside diameter of which being matched with the inside diameter of the guiding hole, was inserted into the hole, passed through detection cell and finally got access to the exit of the separation channel, the electrode could be quasi-self-aligned to the channel exit and fixed inside the guiding hole without the help of a micro-positioner. Thus an integrated end-column amperometric detection system with replaceable disk working electrode was established on the microchip. Good reproducibility of the electrode alignment was demonstrated by a 4 1% RSD in the detection of sucrose after repeated(n=9) alignment of a micro-copper disk electrode. The developed michrochip was used for separation and detection of oligosaccharides.