| Abstract: | Abstract A new receptor assay method for benzodiazepine was developed using biotin-1012S conjugate as a non-isotopic ligand. The concentration of free ligand in the benzodiazepine receptor suspension was determined by an enzymatic solid-phase avidin-biotin binding assay. The competition reaction between the conjugate and diazepam gave a well-defined dose/response curve for diazepam. The receptor assay could also be applied to the determination of an inverse agonist, n-butyl-P-carboline-3 carboxylate. |
