微乳液毛细管电动色谱研究

本文研究了两组混合样的微乳液毛细管电动色谱(MEEKC)分离, 较系统地研究了微乳液的内相, 助表面活性剂及其浓度对电动分离的迁移时间、柱效及时间窗的影响, 并与胶束毛细管电动色谱(MEKC)分离进行了比较, 选用由80mmol/L正辛烷-120mmol/L十二烷基硫酸钠-900mmol/L正丁醇-10mmol/L硼砂组成的微乳液, 样品组分电动色谱迁移时间的RSD<0.8%, 峰面积RSD<3.0%.

Study on microemulsion electrokinetic chromatography

Microemulsion Electrokinetic Chromatography (MEEKC) is a relatively new capillary electrophoresis technique using a microemulsion as a separation carrier. Two kinds of mixture samples were separated by MEEKC and compared with Micellar Electrokinetic Chromatography (MEKC). The effects of microemulsion composition on MEEKC separation were studied. The different core phase showed different migration time, efficiency and time window. The separation was also affacted by core phase and cosurfactant concentration. Using microemulsion consisting of 80mmol/L octane -120mmol/L sodium dodecyl sulphate -900mmol/L 1-butanol -10mmol/L borate, relative standard deviation (RSD) of migration time was less than 0.8% and RSD of peak area was less than 3% (n=6). High separation efficiency can be obtained if the microemulsion composition is properly chosen.