毛细管区带电泳测定纳米粒子粒度分布的研究

建立了一种用毛细管区带电泳测定纳米粒子平均粒径及粒径分布的新方法。该方法通过外标定量法对样品的原始电泳谱图进行定量校正，从而得到样品的粒径分布图及平均粒径。外标定量法包括迁移时间-粒径校正曲线的建立及不同粒径含量校正曲线的建立。该方法所需样品量少,且粒度分布结果具有统计代表性。

Determination of Particle Size Distribution of Nano-Particles by Capillary Zone Electrophoresis

A new method was established for the determination of the size distribution of nano-particles by capillary zone electrophoresis (CZE). The data processing method for acquiring size distribution from primary electropherogram was investigated. The relative standard deviation of migration time was deduced by using dimethyl sulfoxide (DMSO) as an internal standard. Scattering effect of nano-particles was studied and a correction equation was established to correct this influence. The experimental results showed that the correction method was effective. After correction, the measuring results for the mixed samples with different mass ratios of PS50 and PS125 were almost the same with their theoretical values and the relative deviation was reduced from 35% to 5%. This method for the determination of size distribution is satisfactory.