| 高效液相色谱串联质谱法测定饲料中喹烯酮含量 |
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| 引用本文： | 顾亮,徐彦辉,蒋俊树,丁磊.高效液相色谱串联质谱法测定饲料中喹烯酮含量[J].化学分析计量,2010,19(5):52-54. |
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| 作者姓名： | 顾亮 徐彦辉 蒋俊树 丁磊 |
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| 作者单位： | [1]安徽国家农业标准化与监测中心,合肥230051 [2]安徽大学化学化工学院,合肥230039 |
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| 摘 要： | 建立了液相色谱串联质谱法(HPLC/MS/MS)测定饲料中喹烯酮的含量的方法。饲料样品经匀浆、超声提取后经固相萃取柱中性氧化铝Alumia N(1 g,3 mL)]净化,采用HPLC/MS/MS电喷雾电离,正离子模式,多反应监测(MRM)。喹烯酮浓度在0.01～0.2μg/g范围内标准曲线的相关系数大于0.998。在不同添加水平下的回收率为81.9%～111.6%,检出限(LOD)为5μg/kg。
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| 关 键 词： | 喹烯酮 高效液相色谱／质谱法 饲料 |
DETERMINATION OF QUINOCETONE IN ANIMAL FEED SAMPLES BY HIGH PERFORMANCE LIQUID CHROMATORGRAPHY-TANDEM MASS SPECTROMETRY |
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| Gu Liang,Xu Yanhui,Jiang Junshu,Ding Lei.DETERMINATION OF QUINOCETONE IN ANIMAL FEED SAMPLES BY HIGH PERFORMANCE LIQUID CHROMATORGRAPHY-TANDEM MASS SPECTROMETRY[J].Chemical Analysis And Meterage,2010,19(5):52-54. |
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| Authors: | Gu Liang Xu Yanhui Jiang Junshu Ding Lei |
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| Institution: | 1. Anhui Country Agriculture Standardization and Monitor Center, Hefei 230051, China ; 2. Chemistry and Chemical Engineering, Anhui University, Hefei 230039, China) |
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| Abstract: | A high performance liquid chromatography - tandem mass spectrometry method was established for determination of quinotone in animal feed. Animal feed samples were extracted by a homogenater and an ultrasonic cleaner, purified by a solidphase extraction （SPE） cartridges Alumia N （1 g, 3 mL） ] , detected by the positive eleetrospray ionization （ESI ＋ ） method under muhiple reaction monitoring （MRM） mode. The calibration curves were liner （r 〉 0. 998 ） in the concentration range of 0.01- 0.2 μg/g. The extraction recoveries range from 81.9% to 111.6% in different spiked concentrations samples. The LOD was 5 μg/kg. |
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| Keywords: | quinocetone liquid chromatography- tandem mass spectrometry feed |
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