纤维素固定化血红蛋白研究

纤维素是一种重要的化工原料，由于来源广泛、价廉以及良好的再生性、反应性和通用性，被广泛用做载体材料．在酶的分离纯化，蛋白质分离纯化，亲合色谱固定相，拆分手性化合物等应用领域，往往采用球形纤维素或膜状纤维素形式．而在水吸附剂，分离回收金属离子，从海水中...

MECHANISM STUDY OF HEMOGLOBIN IMMOBILIZATION ON PERIODATE OXYCELLULOSE

Cellulose can be easily oxidized to oxycellulose with periodic acid,which can serve as a carrier for the immobilization of hemoglobin acting like a bionic gill,to extract oxygen from sea water,which may provide a source of oxygen for human life support in an undersea environment.The present paper studied the mechanism of hemoglobin immobilization on periodate oxycellulose.It was found that only by the unique activation procedure,that the cotton was first oxidized with NaIO 4,then treated with 5.0mol/L NaOH and again oxidized with NaIO 4,the carrier thus obtained was able to immobilize a very high amount of Hb,i.e.1.0g Hb/g cellulose.When cellulose was oxidized only once with NaIO 4,although the aldehyde group content was rather high,the linking amount of Hb was very low.The mechanism was studied by means of X diffractioin,infrared spectrum and determination of functional groups.It was found that Hb was immobilized by the interaction between the aldehyde groups in the oxycellulose and the amino groups on Hb.And the microcrystalline structure in cellulose was the main hindrance in immobilization process.Alkaline degradation of periodate oxycellulose led to destruction of the microcrystalline structure.The alkaline degradation and Cannizzaro reaction also resulted in a complete loss of aldehyde groups.In order to obtain maximum amount of immobilized Hb,a second oxidation with NaIO 4 was necessary to produce aldehyde groups.