| 固相萃取-高效液相色谱法测定鱼/虾生物体中甲苯咪唑及代谢物的残留 |
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| 引用本文： | 于慧娟,冯兵,姜朝军,张晓玲,惠芸华,沈晓盛.固相萃取-高效液相色谱法测定鱼/虾生物体中甲苯咪唑及代谢物的残留[J].分析试验室,2010,29(4). |
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| 作者姓名： | 于慧娟 冯兵 姜朝军 张晓玲 惠芸华 沈晓盛 |
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| 作者单位： | 1. 中国水产科学研究院东海水产研究所,上海,200090
2. 大连水产学院,大连,116023 |
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| 基金项目： | 农业部国家标准制、修订(2007-204)项目资助 |
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| 摘 要： | 建立了同时测定鱼、虾生物体中甲苯咪唑及代谢物-氨基甲苯咪唑和羟基甲苯咪唑残留的分析方法。样品以水和乙酸乙酯提取,正己烷去脂、MCX固相萃取柱净化,高效液相色谱-紫外检测器测定。重点研究了样品前处理方法,并对各种不同的固相萃取柱的净化效果和准确度进行了研究。甲苯咪唑及代谢物在5～200μg/kg的添加水平下回收率为81.4%～95.3%,相对标准偏差为1.9%～8.6%,方法线性范围为10～2000ng/mL。方法检测限为甲苯咪唑:5μg/kg,羟基甲苯咪唑:5μg/kg,氨基甲苯咪唑:10μg/kg。
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| 关 键 词： | 甲苯咪唑 代谢物 固相萃取 残留 高效液相色谱 |
Determination of residues of mebendazole and its metabolites in fish/shrimp by solid phase extraction-high performance liquid chromatography |
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| YU Hui-juan,FENG Bing,JIANG Chao-jun,ZHANG Xiao-ling,HUI Yunhua,SHEN Xiao-sheng.Determination of residues of mebendazole and its metabolites in fish/shrimp by solid phase extraction-high performance liquid chromatography[J].Chinese Journal of Analysis Laboratory,2010,29(4). |
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| Authors: | YU Hui-juan FENG Bing JIANG Chao-jun ZHANG Xiao-ling HUI Yunhua SHEN Xiao-sheng |
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| Institution: | 1.East China Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Shanghai 200090;2.DaLian Fisheries University;Dalian 116023 |
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| Abstract: | An analytical method was developed to detect residues of mebendazol and its metabolites in fish and shrimp with high performance liquid chromatography-UV detector.The mebendazol and its metabolites were extracted with water and ethyl acetate,defatted with hexane,and purified on a MCX solid phase extraction column.It was studied by sample pre-treating method.The purification efficiency and accurancy of solid phase extraction columns were studied.The recoveries of mebendazol and its metabolites were in the ra... |
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| Keywords: | Mebendazol Metabolites Solid-phase extraction Residue High Performance liquid chromatography |
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