| 聚乙烯亚胺改性聚丙烯腈中空膜固定脂肪酶 |
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| 引用本文： | 卢佳伟,李由然,石贵阳.聚乙烯亚胺改性聚丙烯腈中空膜固定脂肪酶[J].分子催化,2018,32(1):79-89. |
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| 作者姓名： | 卢佳伟 李由然 石贵阳 |
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| 作者单位： | 江南大学 生物工程学院, 江苏 无锡 214122;江南大学 粮食发酵工艺与技术国家工程实验室, 江苏 无锡 214122,江南大学 生物工程学院, 江苏 无锡 214122;江南大学 粮食发酵工艺与技术国家工程实验室, 江苏 无锡 214122,江南大学 生物工程学院, 江苏 无锡 214122;江南大学 粮食发酵工艺与技术国家工程实验室, 江苏 无锡 214122 |
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| 基金项目： | 国家自然科学基金青年基金项目（No.31401674）（National Natural Science Foundation of China （No.31401674））;"十三五"国家重点研发计划项目（No.2016YFD0401400） |
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| 摘 要： | 聚丙烯腈是富腈基的高分子聚合物,易修饰改性,广泛应用于膜分离应用.我们以聚丙烯腈中空膜为载体,采用化学法交联聚乙烯亚胺并固定脂肪酶,固定过程中引入海藻酸钠,用CaCl_2进行后处理,得到固定化脂肪酶PAN-PEI-SA/E-CaCl_2载酶量为31.70(mg enzyme)/(g support),酶活为50.20 U/(g support),15次重复使用可保留58.77%的酶活,与游离酶相比耐酸性和耐温性有所提高,相同条件下与Nov 435相比,酶活更高,这表明最终得到的固定化脂肪酶有良好的工业应用前景.
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| 关 键 词： | 聚丙烯腈 表面改性 酶固定化 脂肪酶 |
| 收稿时间： | 2017/11/20 0:00:00 |
| 修稿时间： | 2017/12/20 0:00:00 |
The Modification of Polyacrylonitrile Hollow Membrane by Polyethyleneimine to Immobilize Lipase |
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| LU Jia-wei,LI You-ran and SHI Gui-yang.The Modification of Polyacrylonitrile Hollow Membrane by Polyethyleneimine to Immobilize Lipase[J].Journal of Molecular Catalysis (China),2018,32(1):79-89. |
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| Authors: | LU Jia-wei LI You-ran and SHI Gui-yang |
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| Institution: | School of Biotechnology, Jiangnan University, Wu xi, 214122, China;National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wu xi 214122, China,School of Biotechnology, Jiangnan University, Wu xi, 214122, China;National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wu xi 214122, China and School of Biotechnology, Jiangnan University, Wu xi, 214122, China;National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wu xi 214122, China |
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| Abstract: | Polyacrylonitrile is a nitrile-rich polymer and easy to be surface modified, and it has been widely applied in membrane separation. In this study, polyacrylonitrile hollow membrane was used as a support of enzyme immobilization by chemically cross-linking polyethyleneimine. Sodium alginate was added during immobilization, and the immobilized lipase was post-treated by CaCl2. The amount of loading enzyme of PAN-PEI-SA/E-CaCl2 was 31.70 (mg enzyme)/(g support) and the activity as 50.20 U/(g support).After 15 cycles of reactions, the immobilized enzyme retained 58.77% of initial enzyme activity, and showed an improvement of heat and acid resistance compared to free lipase. When it is compared to commercialized Nov 435 under same condition, PAN-PEI-SA/E-CaCl2 has higher enzyme activity during 15 cycles of reactions. It shows the immobilized lipase with PAN after surface modification has industrial application prospects. |
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| Keywords: | polyacrylonitrile surface modification enzyme immobilization lipase |
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