Isolation of lipase producer and its performance in enantioselective hydrolysis of glycidyl butyrate |
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| Authors: | Jia Shi-Ying Xu Jian-He Li Qing Shan Yu Jun Tang |
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| Institution: | (1) Laboratory of Biocatalysis, PO Box 283, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, P. R. China |
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| Abstract: | A racemic glycidyl butyrate resolving strain, preliminarily identified as a Rhizopus sp., had been isolated from soil. Its extracellular lipase was found to enantioselectively hydrolyze the (S)-enantiomer of
the chiral ester, with optimal activities at pH 5.3 and 42°C. Higher en antioselectivity of theenzyme was observed at lower
temperatures, while the best anantioselectivity was obtained at pH 5.5–6.0, with an, E value (enantiomeric ratio) of 57. |
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| Keywords: | (R S )-Glycidyl butyrate optical resolution lipase Rhizopus sp isolation |
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