Loss of PEG chain in routine SDS‐PAGE analysis of PEG‐maleimide modified protein

SDS‐PAGE represents a quick and simple method for qualitative and quantitative analysis of protein and protein‐containing conjugates, mostly pegylated proteins. PEG‐maleimide (MAL) is frequently used to site‐specifically pegylate therapeutic proteins via free cysteine residue by forming a thiosuccinimide structure for pursuing homogeneous products. The C–S linkage between protein and PEG‐MAL is generally thought to be relatively stable. However, loss of intact PEG chain in routine SDS‐PAGE analysis of PEG‐maleimide modified protein was observed. It is a thiol‐independent thioether cleavage and the shedding of PEG chain exclusively happens to PEG‐MAL modified conjugates although PEG‐vinylsulfone conjugates to thiol‐containing proteins also through a C–S linkage. Cleavage kinetics of PEG40k‐MAL modified ciliary neurotrophic factor showed this kind of degradation could immediately happen even in 1 min incubation at high temperature and could be detected at physiological temperature and pH, although the rate was relatively slow. This may provide another degradation route for maleimide‐thiol conjugate irrespective of reactive thiol, although the specific mechanism is still not very clear for us. It would also offer a basis for accurate characterization of PEG‐MAL modified protein/peptide by SDS‐PAGE analysis.     

环烷酸对油水界面膜流变性质的影响研究

研究了环烷酸对油水界面膜界面张力、弹性模量、损耗模量以及界面膜破裂速率常数的影响,同时对环烷酸与沥青质之间的相互作用进行了测定。结果表明,环烷酸使得原油油水界面张力下降;弹性模量随着环烷酸加量以及振荡频率的增加都分别逐渐增大,并且最终都趋于平衡;在任何振荡频率值时,损耗模量都随着环烷酸加量先增大后减小;当环烷酸加量增加时,界面膜破裂速率常数降低。环烷酸与沥青质之间存在相互作用,随着环烷酸加量的增加,其对沥青质界面膜弹性模量的影响与对原油界面膜弹性模量的影响相似,表明环烷酸主要是通过与沥青质相互作用而促进乳状液稳定性的。     

基于CdSe/ZnS量子点薄膜结构的高双折射光子晶体光纤的色散与损耗控制

基于有限元法,设计了一种六边形排列含CdSe/ZnS量子点薄膜结构的高双折射光子晶体光纤,分析了具有不同厚度CdSe/ZnS量子点薄膜光子晶体光纤的色散及损耗特性.结果表明,含CdSe/ZnS量子点薄膜结构的光子晶体光纤在x和y方向均存在基模.当泵浦光波长逐渐增加时,具有相同厚度CdSe/ZnS量子点薄膜光子晶体光纤的双折射值逐渐增大,x和y方向总色散先增大后减小且存在两个零色散点,损耗逐渐增大并在可见光波段趋近于零;具有不同厚度CdSe/ZnS量子点薄膜光子晶体光纤随CdSe/ZnS量子点薄膜厚度的增加,在相同泵浦光波长处,双折射值逐渐减小,x和y方向总色散逐渐减小且两个零色散点逐渐靠近,损耗逐渐增大.通过沉积不同厚度CdSe/ZnS量子点薄膜和选择合适泵浦光波长,可有效控制光子晶体光纤的色散和损耗.     

Spherically Averaged Endpoint Strichartz Estimates For The Two­Dimensional Schrödinger Equation

The endpoint Strichartz estimates for the Schrödinger equation is known to be false in two dimensions[7]. However, if one averages the solution in L2 in the angular variable, we show that the homogeneous endpoint and the retarded half­endoint estimates hold, but the full retarded endpoint fails. In particular, the original verisions of these estimates hold for radial data     

高真空多层绝热容器真空丧失后传热研究综述

高真空多层绝热低温容器突然的真空丧失可能会引发重大的安全事故.文中综述了国内外研究者在高真空多层绝热低温容器突然的真空丧失方面的研究成果,并对未来的研究方向进行了讨论和展望.     

Energy losses of ice slurry in pipe sudden contractions

The paper presents the results of the experimental research on the ice slurry loss coefficient during its flow through sudden contractions. Experimental studies were conducted using a few of the most common contractions of copper pipes. Six contraction ratios were covered: 0.500, 0.615, 0.650, 0.769, 0.800 and 0.813. In the experimental research, the mass fraction of solid particles in the slurry ranged from 20 to 30 (20%, 25%, 30%). Research results allow for determining the theoretical correlation for the ice slurry, in order to calculate the loss coefficient in contractions during laminar flow. The results of experimental investigations also confirmed that the loss coefficients in contractions in turbulent (transitional) flow of the ice slurry are the same as in the case of Newtonian liquids. The paper also presents original theoretical correlation for calculating the kinetic energy correction factor in the laminar range flow of liquid in the Bingham model. This correlation is used in this work for calculation of the ice slurry flow through contractions.     

The decision to raise firm value through a sports-business exchange: How much are Real Madrid’s goals worth to its president’s company’s goals?

Global brands emerging from the world of sports are becoming commonplace, and firms invest in the realm of sports, usually through sponsorship initiatives, to get a link with these global brands. Over and above just a mere business link, what if a company makes a personal commitment to get into the core of a renowned, celebrated sports team? This article provides managers with a procedure to analyze, in a weekly basis, how valuable this type of decision is. A conceptual model shows that the personal involvement of a firm’s figurehead in a first-class sports club can impact positively on firm value if the person is doing well in the task s/he is entrusted with by the club. The empirical application to the soccer club Real Madrid, over 1,409 days and 215 matches, finds that the club’s performance on the field has a significant impact on the economic returns of its president’s company, with asymmetrical effects on firm value in a “loss aversion” pattern, that is, lost matches have a greater effect on firm value than games won.     

Optimal investment of DC pension plan under short-selling constraints and portfolio insurance

In this paper we investigate an optimal investment problem under short-selling and portfolio insurance constraints faced by a defined contribution pension fund manager who is loss averse. The financial market consists of a cash bond, an indexed bond and a stock. The manager aims to maximize the expected S-shaped utility of the terminal wealth exceeding a minimum guarantee. We apply the dual control method to solve the problem and derive the representations of the optimal wealth process and trading strategies in terms of the dual controlled process and the dual value function. We also perform some numerical tests and show how the S-shaped utility, the short-selling constraints and the portfolio insurance impact the optimal terminal wealth.