原子荧光法测定甘草中汞

利用还原气化的非色散型装置，以湿法消解的方式，对中药甘草中的痕量汞进行了原子荧光测定。研究了影响测定结果的条件，比较了两种玻璃仪器的洗涤方式，并以工作曲线法和加入作图法对照，试验回收率在86．9％－110．8％，误差＜13．1％，得出了满意的分析结果。     

Rapid all-in-one three-step immunoassay for non-instrumental detection of ochratoxin A in high-coloured herbs and spices

A feasible three-step method for ochratoxin A (OTA) rapid detection was developed and applied for OTA screening in high-coloured matrices such as liquorice, ginger, nutmeg, black pepper, white pepper and Capsicum spp. spices at a control level of 10 μg kg⁻¹. The method was based on the clean-up tandem immunoassay column and involved three steps: extract application, washing step and application of chromogenic substrate. A significant simplification of the assay was reached by using an additional frit with conjugate inside the clean-up tandem immunoassay column. The time for analysis was less than 10 min, including 5 min for colour development. Results were visually evaluated as colour development for negative result or no colour development for positive result. The method was coupled with a simple methanol-based extraction. A total of 27 samples were screened for OTA with the proposed method. It was shown that two samples of red pepper and one sample of liquorice, pili-pili, chilli and cayenne were contaminated with OTA above the control level at 10 μg kg⁻¹, but none of tested ginger, nutmeg, black pepper and white pepper.     

火焰原子吸收光谱法测定甘草中的铁、锌和锰

采用火焰原子吸收光谱法测定了甘草中的微量元素铁、锌和锰的含量 ,方法简单 ,精密度和灵敏度高 ,相对标准偏差为 0 .2 36 %— 0 .718% ,回收率为 94 .5 %— 99.2 %。     

固相萃取技术-HPLC测定复方甘草片中吗啡的含量

报道了用ＳＥＰ－ＰＡＫＣ１８小预柱固相萃取技术和反相高效液相色谱法测定复方甘草片中微量吗啡含量的方法。对固相萃取条件进行了研究,最终选择１０％甲醇溶液为清洗溶液,７０％甲醇溶液为洗脱溶液,基本消除了干扰组分对测定的影响,色谱图背景吸收小,基线平稳。色谱柱为μ－ＢｏｎｄａｐａｋＣ１８柱,流动相为０．１ｍｏｌ／ＬＮａＨ２ＰＯ４溶液－甲醇（５１）,检测波长为２８６ｎｍ。平均回收率为（１０１．２±１．５）％,ＲＳＤ为１．５％。