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通过发根农杆菌的转化作用,在甘蓝、油菜和花椰菜幼苗的下胚轴切段上诱发产生了许多Ri T-DNA转化根.用根肿菌休眠孢子悬浮液接种转化根,分别在固体、液体和半固体MS培养基上培养. 在固体培养基上,根肿菌只侵入根毛内,不侵入根皮层组织,因而不表现根肿病的任何症状.在液体培养基内,根肿菌侵入根皮层,导致根皮层组织异常的增生和膨大,直至形成肿根症状.在半固体培养基内,根肿菌侵入根段切口处的细胞内,并很快使切口处膨大起来. 相似文献
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Rong Min Yu Liang Bin Zhou Chun Yan Yan Guo Yan Duan Yu Zhao 《中国化学快报》2008,19(1):76-78
The glycosylation of hydroxylcoumarin was investigated by using suspension cultures of hairy roots of Polygonum multiflorum. Two new coumarin glucosides (3 and 4) were biosysthesized by regioselectively glycosylation of corresponding substrates (1 and 2) in the system. The structures of two products were identified as 7-hydroxy-4-methylcoumarin 5-O-β-D-glucopyranoside and 7- hydroxy-3,4-dimethylcoumarin 5-O-β-D-glucopyranoside on the ground of chemical and spectroscopic methods, respectively. 2007 Rong Min Yu. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved. 相似文献
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银杏发根的转化及其悬浮培养无性系的建立 总被引:2,自引:0,他引:2
运用具有Ri质粒的发根农杆菌对银杏的叶片、幼芽和幼茎进行成功地转化,并建立银杏发根的悬浮培养无性系.冠瘿碱检测实验表明,发根农杆菌Ri质粒的T-DNA部分已整合到银杏细胞中.这为利用生物技术开发银杏资源提供一条新的途径. 相似文献
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In this article, the authors discuss two kinds of new planar maps: pan-fan maps and circuit boundary maps, and provide explicit expressions about their enumerating functions with different parameters. Meanwhile, two explicit counting formulas for circuit cubic boundary maps with two parameters; the size and the valency of the root-face, are also extracted. 相似文献
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