有限体积KFVS方法在二维溃坝中的应用

本文采用了基于KFVS格式的有限体积方法 (FVM)求解了控制水流运动的二维浅水方程 ,建立了二维水坝瞬间溃坝的洪水演进模型 .并应用此模型模拟了二维非对称溃坝和对称溃坝情形下坝左下角有障碍物时的洪水波演进过程 .模拟结果表明该数学模型对二维浅水运动的模拟很有效 .     

High Sensitive Electrochemiluminescence Biosensor Based on Ru(phen)32+-loaded Double Strand DNA as Signal Tags use to Detect DNA Methyltransferase Activity

DNA methyltransferase (DNA MTase) can act as biomarker for many diseases and it is important to develop some new methods for sensitive detection of DNA MTase. In this work, a highly efficient electrochemiluminescence (ECL) sensor had been designed for detection of DNA MTase based on Ru(phen)₃²⁺ loaded double strand DNA (dsDNA- Ru(phen)₃²⁺) as signal tags. Ru(phen)₃²⁺ had been efficiently embed in the dsDNA produced through a simple hybridization chain reaction. First, a hairpin probe was designed, which can be specifically recognized by Dam MTase and modified with -SH at one end. It was modified on the surface of gold electrode by -SH as an immobilization probe (IP). This IP will be methylated in the present of Dam MTase and digested by DpnI following. Results in the release of capture probe (CP) which remains on the surface of gold electrode. The CP can hybridize with the single stand part of the dsDNA- Ru(phen)₃²⁺ and make the immobilization of ECL tags on the electrode surface, which results in a strong ECL signals detected. However, without the effect of Dam MTase, the hairpin structure of IP remains stable and cannot capture signal tags, and can only detecte weak ECL signals. The biosensor can detect the activity of Dam MTase in the concentration range of 0.01 U/mL to 20 U/mL with the ECL intensity and the logarithm of the concentration have a linear relationship, and the detection limit is calculated to be 7.6 mU/mL. The developed sensor has the ability to specifically detect Dam MTase, which can be differentiated from other types of DNA MTase. In addition, the designed method has good applicability to detect Dam MTase activity in serum samples and been applied to detect its inhibitor with high efficiency.     

A free boundary problem for a fluid flow in a heterogeneous porous medium

In this paper we study the problem of seepage of a fluid through a porous medium, assuming the flow governed by a nonlinear Darcy law and nonlinear leaky boundary conditions. We prove the continuity of the free boundary and the existence and uniqueness of minimal and maximal solutions. We also prove the uniqueness of theS ₃-connected solution in various situations.     

Food Web Implications of δ13C and δ15N Variability over 370 km of the Regulated Colorado River USA

Abstract

Dual stable isotope analysis in the regulated Colorado River through Grand Canyon National Park, USA, revealed a food web that varied spatially through this arid biome. Down-river enrichment of δ¹³C data was detected across three trophic levels resulting in shifted food webs. Humpack chub δ¹³C and δ¹⁵N values from muscle plugs and fin clips did not differ significantly. Humpback chub and rainbow trout trophic position is positively correlated with standard length indicating an increase in piscivory by larger fishes. Recovery of the aquatic community from impoundment by Glen Canyon Dam and collecting refinements for stable isotope analysis within large rivers are discussed.     

某大型水电枢纽坝基埋藏型岩溶发育特征及深层承压水研究

作者在现场工程地质勘察、试验基础上, 对我国西南某大型水电枢纽坝基所遇特殊的复杂埋藏型岩溶、承压水问题进行了研究, 弄清了坝基岩溶、承压水形成条件及发育特性, 对坝址选择及工程处理的决策具有重要意义。     

新疆下坂地水库坝基防渗墙试验研究

新疆下坂地水利枢纽工程地处高寒、高海拔的山区,坝基覆盖层厚度达147.95m,透水性强,易坍塌和漏浆,块石坚硬,坝基防渗处理难度罕见。针对这一工程问题进行了坝基防渗墙试验研究,结果表明,以大深度混凝土防渗墙为主墙,下接帷幕灌浆的墙幕结合方案。本文针对新疆下坂地深厚覆盖层坝基防渗墙进行了试验研究,找出了防渗墙有效的施工深度及合理的施工方法,从而为坝基防渗墙设计施工提供了可靠的依据。     

清江隔河岩坝基工程岩体质量评价研究

采用《工程岩体分级标准》定性和定量相结合的方法,根据岩石坚硬程度和岩石完整程度决定岩体的基本质量,然后考虑地下水状态、初始应力状态、工程轴线方位与主要软弱结构面产状的组合关系等修正因素,确定坝基工程岩体的级别。针对隔河岩工程考虑岩溶、层间剪切带、应力水平、岩体所处工程部位等因素,给出隔河岩工程坝基工程岩体分级。     

对蓬安县三元水库大坝外坡裂缝产生的原因及整治工程的探讨

本文通过对蓬安县三元水库大坝外坡裂缝产生的原因分析，进而运用岩、土力学的理论与方法反算推论出了裂缝的产生不是深层坝体土滑动引起的，而是局部浅层坝体土在水的诱发作用下作蠕滑-拉裂变形所产生的。在明确了大坝裂缝产生的原因后，提出了一系列相应整治工程措施，以达到彻底根治裂缝和稳固大坝的目的。     

高精度激光倾角测量技术研究

本文提出了一种高精度激光电子二维倾角测量技术,该技术利用液体表面多次光反射,将倾角变化转换成激光光斑的位移,并通过多次光学反射实现光学放大,从而实现了倾角和倾角变化的高精度测量.利用本技术实现的倾角测量仪器具有测量精度高、体积小等优点,经过标定试验系统测量精度达到2.5″.高精度激光电子二维倾角测量系统可广泛应用于水坝安全监测工程、各种建筑和检测领域.     

Experimental investigation of dynamic pressure loads during dam break

The objective of this research work has been to conduct experimental measurements on a dam break flow over a horizontal dry bed in order to provide a detailed insight, with emphasis on the pressure loads, into the dynamics of the dam break wave impacting a vertical wall downstream the dam. The experimental setup is described in detail, comprising state of the art miniaturized pressure sensors, high sampling rate data acquisition systems and high frame-rate video camera. It is a 1:2 scale of the highly cited (Lee et al., 2002, Journal of Fluids Engineering, 124) article experimental apparatus. Kinematics has been analyzed focusing on the free surface and wave front evolution. Experimental observations regarding liquid height and wave front speed have found to be in agreement with existing literature. This agreement enables the authors, assuming a similar framework, to discuss the measured pressure loads as a consequence of the dam break wave front impacting on the downstream wall. These loads show a substantial variability which has been statistically characterized. The measured quantities have been compared with the scarce available data in the literature, whose consistency is discussed. Measurements have been conducted with two filling heights. Scaling effects for such heights are also analyzed. As a direct result of the present initiative, an extensive set of data for computational tools validation is provided as Supplementary Materials, including pressure signals, wave height measurements and experimental videos.