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1.
The modifying action of chloroform on photochemical reactions induced in the eye lenses of rats by UVB radiation is revealed. In anesthetic concentrations, halogenmethane increases the quantum yield of photodisintegration of protein tryptophanyls with formation of a new photochemical product that absorbs radiation in the UVA range and possesses the photosensitizing activity. 相似文献
2.
In this work actin is used to illustrate connection of protein fluorescence characteristics with its structure. On one hand, it has been demonstrated what kind of information about the contribution of each tryptophan residues to the bulk fluorescence spectrum can be obtained from the special analysis of protein three-dimensional structure. On the other hand, potentials of intrinsic fluorescence for elucidation of proteins structure, dynamics and processes of folding-unfolding are shown. In particular, using this method a new essentially unfolded kinetic intermediate state of actin was detected and characterized, and the place of inactivated actin and its kinetic predecessor in the process of folding-unfolding was determined. It has been revealed that inactivated actin is not intermediate state between the native and completely unfolded states, as it has been accepted before, but a result of protein misfolding. On the basis of the obtained data a new model of actin folding-unfolding pathway has been proposed. 相似文献
3.
酸糖蛋白手性柱分离6种手性化合物 总被引:3,自引:0,他引:3
通过考察缓冲液种类、浓度及其pH值对对映体在手性柱上的保留和分离行为的影响,以及流动相中加入不同种类、不同浓度的不带电荷的有机溶剂乙腈、甲醇、正丙醇、异丙醇、流动相流速和柱温对对映体分离能力的影响,优化了含碳手性中心的碱性药物苯丙哌林、酸性化合物MT-A5及MT-酸和含硫手性中心的质子泵抑制剂奥美拉唑、泮托拉唑、雷贝拉唑对映体分离条件,最佳手性分离条件为:苯丙哌林,0.05 mol/L磷酸二氢铵缓冲液(pH 3.0)-乙腈(95∶5,V/V)为流动相,流速为0.7 mL/m in,柱温为20℃;MT-A5及MT-酸,流动相为0.01 mol/L醋酸铵缓冲液(pH 5.0)-乙腈(74∶26,V/V),流速为0.9 mL/m in,柱温为20℃;泮托拉唑,流动相为10 mmol/L醋酸铵缓冲液(pH 5.5)-乙腈(93∶7,V/V),流速为0.9 mL/m in;柱温为20℃;奥美拉唑和雷贝拉唑,流动相为0.01 mol/L醋酸铵缓冲液(pH 3.0)-乙腈(95∶5,V/V),流速为0.7 mL/m in,柱温为20℃。实现了应用高效液相色谱法在α1-酸糖蛋白手性柱上对上述化合物的对映体分离,并成功用于手性药物合成中的对映体过量百分率的测定。 相似文献
4.
Detailed pH-dependent steady state and picosecond time-resolved tryptophan fluorescence studies on thiocyanate and azide complexes
of horseradish peroxidase have been carried out. The fluorescence decay of the single tryptophan in these species was fitted
to a discrete three exponential model. Maximum entropy method analysis also gave three distinct regions of lifetime distributions.
The fast subnanosecond lifetime component was found to have > 97% amplitude contribution while other two longer lifetime components
have small contributions. Small contributions from the nanosecond lifetime components possibly arise from apoprotein impurity
or some small amount of disordered heme conformer of the protein. pH dependence of the fast picosecond lifetime components
was found to show a systematic behavior which has been interpreted in the light of obligatory conformation change associated
with activation of the enzyme at low pH. 相似文献
5.
M. Wesolowski 《Journal of Thermal Analysis and Calorimetry》1992,38(9):2239-2245
A short survey is given of some aspects of the application of thermoanalytical methods, especially differential thermal analysis (DTA), differential scanning calorimetry (DSC) and thermogravimetry (TG), in solid-dosage technology. The usefulness of these methods in the prediction of drug-excipient compatibility, studies of solid-dispersion systems, the analysis of enantiomers and racemates, measurement of the time of tablet disintegration, the analysis of drug formulations and studies of the processes of grinding and drying of drugs is discussed.
Zusammenfassung Es wird ein kurzer Überblick über einige Aspekte der Anwendung von thermoanalytischen Methoden, insbesondere von DTA, DSC und TG in der Feststoffdosierung gegeben. Weiterhin wird die Nützlichkeit dieser Methoden bei der Voraussage der Bindemittelverträglichkeit, bei der Untersuchung disperser Feststoffsysteme, bei der Analyse von Enantiomeren und Racematen, bei der Messung der Tablettenzerfallszeit, bei der Analyse der Arzneimittelformierung und bei der Untersuchung von Mahl- und Trocknungsprozessen bei Arzneimitteln diskutiert.相似文献
6.
Summary Chiral recognition of many enantiomeric solutes by a chiral amide stationary phase is based mainly on hydrogen bonding. A
chiral-recognition-factor CHI is proposed, given by the difference of the enthalpy change in the enantiomer discrimination,
standardized with respect to the specific interaction of the solutes with the diamide core of the stationary phase. The r?le
of the entropy part is also discussed. By extrapolation of the retention behaviour to elevated temperature, peak inversion
of enantiomers is predicted.
Presented at the 15th International Symposium on Chromatography, Nürnberg, October 1984 相似文献
7.
手性配体立体选择性萃取分离扁桃酸对映体 总被引:6,自引:0,他引:6
Based on chiral ligand exchange, the distribution behavior of mandelic acid enantiomers in the two-phase system containing copper ion(Ⅱ)(Cu2+) and N-n-dedecane-L-hydroxyproline (L), was studied. At different pH, the partition of Cu2+ in the two-phase system containing N-n-dedecane-L-hydroxyproline, was investigated. The formation constant of binary cpmplex(Li2Cu) was obtained by regression. The influence of pH, concentrations of Cu2+ and chiral ligand, and solvents on partition coefficients(K) and separation factor(α), was discussed. The experimental results show that Li forms more stable ternary complex with D-mandelic acid than with L- mandelic acid. There is a large influence of pH on K and α. At low pH values (<3.5), formation of binary complexes is thermodynamically unfavourable. K and α are best when pH values are above 3.5 and the molar ratio of chiral ligand to Cu2+ is 2∶1. At the same time, solvents influence K and α very much. 相似文献
8.
9.
Summary Chiral stationary phases (CSPs) for liquid chromatography were prepared by the way of an activated carbamate intermediate.
The amino group of aminopropylsilyl silica gel was first activated by carbamylation with disuccinimido carbonate (DSC). The
obtained activated carbamate silica gel (ACsil) proved useful as an intermediate for the preparation of urea-type CSPs. The
reaction of ACsil with (S)- of (R)-1-(α-naphthyl)-ethylamine gave naphthylethylurea type CSPs. These CSPs were also obtained
directly from aminopropylsilyl silica gel by its reaction with optically active (S)- or (R)-succinimido 1-(α-naphthyl)ethyl
carbamate (SINEC). Several phenylthiohydantoin amino acid enantiomers and p-bromophenylcarbamyl amino acid enantiomers were
resolved on the CSPs by elution with aqueous mobile phase. 相似文献
10.
Summary A fast and simple HPLC-method for the determination of synthetic amino acids in adulterated orange juice has been developed. The amino acid enantiomers were derivatized with a chiral reagent and the derivatives separated on a 3 m particle size C18 column. An electrochemical detector operating in the oxidative mode was used for detection. The potential at which the derivatives are oxidized was determined by cyclic voltammetry.By using selective (electrochemical) detection it is possible to reduce the sample clean-up to simple centrifugation and filtration steps. 相似文献