Unsteady axisymmetric stagnation flow on a circular cylinder

Unsteady, axisymmetric stagnation flow about a circular cylinderis examined when the far-field flow is a periodic function oftime with a fixed time average and an oscillatory part of prescribedamplitude and frequency. Solutions are computed for arbitraryvalues of the Reynolds number, quantifying the effects of surfacecurvature, and a frequency parameter based on the period ofthe far-field flow. It is found that solutions remain regularand periodic provided that the far-field amplitude lies belowa critical value. Above this value, solutions terminate in afinite-time singularity. The blow-up time is delayed by increasingthe curvature of the surface. These results are corroboratedby asymptotic predictions valid in the limits of small and largeamplitude and frequency. For large Reynolds number, the problemreduces to the two-dimensional stagnation-point flow againsta plane wall studied by previous authors.     

Mass Activated Droplet Sorting (MADS) Enables High‐Throughput Screening of Enzymatic Reactions at Nanoliter Scale

Microfluidic droplet sorting enables the high‐throughput screening and selection of water‐in‐oil microreactors at speeds and volumes unparalleled by traditional well‐plate approaches. Most such systems sort using fluorescent reporters on modified substrates or reactions that are rarely industrially relevant. We describe a microfluidic system for high‐throughput sorting of nanoliter droplets based on direct detection using electrospray ionization mass spectrometry (ESI‐MS). Droplets are split, one portion is analyzed by ESI‐MS, and the second portion is sorted based on the MS result. Throughput of 0.7 samples s^?1 is achieved with 98 % accuracy using a self‐correcting and adaptive sorting algorithm. We use the system to screen ≈15 000 samples in 6 h and demonstrate its utility by sorting 25 nL droplets containing transaminase expressed in vitro. Label‐free ESI‐MS droplet screening expands the toolbox for droplet detection and recovery, improving the applicability of droplet sorting to protein engineering, drug discovery, and diagnostic workflows.     

Photon Equivalents as a Parameter for Scaling Photoredox Reactions in Flow: Translation of Photocatalytic C−N Cross‐Coupling from Lab Scale to Multikilogram Scale

With the development of new photocatalytic methods over recent decades, the translation of these chemical reactions to industrial‐production scales using continuous‐flow reactors has become a topic of increasing interest. In this context, we describe our studies toward elucidating an empirically derived parameter for scaling photocatalytic reactions in flow. By evaluating the performance of a photocatalytic C?N cross‐coupling reaction across multiple reactor sizes and geometries, it was demonstrated that expressing product yield as a function of the absorbed photon equivalents provides a predictive, empirical scaling parameter. Through the use of this scaling factor and characterization of the photonic flux within each reactor, the cross‐coupling was scaled successfully from the milligram scale in batch to a multi‐kilogram reaction in flow.     

Polyethylene glycol matrix reduces the rates of photochemical and thermal release of nitric oxide from S-nitroso-N-acetylcysteine

S -nitrosothiols have many biological activities and may act as nitric oxide (NO) carriers and donors, prolonging NO half-life in vivo. In spite of their great potential as therapeutic agents, most S -nitrosothiols are too unstable to isolate. We have shown that the S -nitroso adduct of N -acetylcysteine (SNAC) can be synthesized directly in aqueous and polyethylene glycol (PEG) 400 matrix by using a reactive gaseous (NO/O₂) mixture. Spectral monitoring of the S–N bond cleavage showed that SNAC, synthesized by this method, is relatively stable in nonbuf-fered aqueous solution at 25°C in the dark and that its stability is greatly increased in PEG matrix, resulting in a 28-fold decrease in its initial rate of thermal decomposition. Irradiation with UV light (λ= 333 nm) accelerated the rate of decomposition of SNAC to NO in both matrices, indicating that SNAC may find use for the photogeneration of NO. The quantum yield for SNAC decomposition decreased from 0.65 ± 0.15 in aqueous solution to 0.047 ± 0.005 in PEG 400 matrix. This increased stability in PEG matrix was assigned to a cage effect promoted by the PEG microenvironment that increases the rate of geminated radical pair recombination in the homolytic S–N bond cleavage process. This effect allowed for the storage of SNAC in PEG at −20°C in the dark for more than 10 weeks with negligible decomposition. Such stabilization may represent a viable option for the synthesis, storage and handling of S -nitrosothiol solutions for biomedical applications.     

Application of similitude to soil-machine systems

Model tests can be used to predict the performance of full-scale off-road vehicles and earth-moving and agricultural machines as an aid to their design and development. The principles of similitude as applied to soil-machine system modeling are discussed, and some past studies and examples of these applications are examined. The opportunities for future research to improve these techniques are identified.     

A mixed integer programming model for scheduling orders in a steel mill

The problem of scheduling orders at each facility of a large integrated steel mill is considered. Orders are received randomly, and delivery dates are established immediately. Each order is filled by converting raw materials into a finished saleable steel product by a fixed sequence of processes. The application of a deterministic mixed integer linear programming model to the order scheduling problem is given. One important criterion permitted by the model is to process the orders in a sequence which minimizes the total tardiness from promised delivery for all orders; alternative criteria are also possible. Most practical constraints which arise in steelmaking can be considered within the formulation. In particular, sequencing and resource availability constraints are handled easily. The order scheduling model given here contains many variables and constraints, resulting in computational difficulties. A decomposition algorithm is devised for solving the model. The algorithm is a special case of Benders partitioning. Computational experience is reported for a large-scale problem involving scheduling 102 orders through ten facilities over a six-week period. The exact solution to the large-scale problem is compared with schedules determined by several heuristic dispatching rules. The dispatching rules took into consideration such things as due date, processing time, and tardiness penalties. None of the dispatching rules found the optimal solution.     

Evaluation of the vibrational modes of the human skull as it relates to bone-conducted sound

Analytic and numerical models are used to study bone-conducted sound and how it relates to the vibrational modes of the human skull. The analytic model is based on the solution to the acoustic and elastic wave equations and the constraining boundary conditions for a fluid-filled elastic sphere. Both models predict that most of the acoustic energy of bone-conducted sound exists in the form of surface wave vibrations at the interface between two acoustic media rather than in the bone or cranial chamber. These surface waves have phase speeds much slower than the bulk sound speed for bone. The analytic model, based on spherical elastic shells, predicts a phase speed of 775 m/s and the first resonance frequency at 1500 Hz while the numerical solution yields approximate phase speeds of 450 m/s and provides a visual display of the surface waves and diffraction effects.     

广义Bethe树上奇偶马尔可夫链场上的若干极限性质

通过构造两个非负鞅证明了一个强极限定理,然后把它应用到本文所定义的广义Bethe树上的奇偶马尔可夫链场上,从而获得了此马氏链场上的一类强极限定理.