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本文用视网膜神经节细胞诱向因子(RGNTF)单抗对生后不同龄大鼠视系统的RGNTF进行免疫组化定位、定量。结果发现:在0—1天龄组,大鼠上丘浅,深部的神经细胞及视膜神经节细胞(RGC)呈强阳性,其RGNTF含量分别为88%,100%和100%;在5—6天龄组,其RGNTF含量明显减少,至50%,30%和80%等;随年龄增第RGNTF含量渐减至0%(2年)。用~(32)P标合成RGNTF N-末端7个氨基酸序列的特异性DNA探针作原位杂交。其结果显示:只有0—1天龄组,大鼠上丘浅、深部神经细胞内有强杂交反应,表示大量RGNTF mRNA的存在;而RGC则无杂交反应。结论:初生大鼠的RGC是依赖上丘神经细胞供应RGNTF;其自然死亡可能与上丘的RGNTF大量减产,减少供应有关。 相似文献
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The monoclonal antibody specific to the retinal ganglion neuronotrophic factor (RGNTF) was used to localize and quantify the presence and amount of RGNTF during the postnatal development of the visual system in the rat.The results showed that in the 0-1-day age group, neurons at the superficial layers and deep part of superior colliculus, and as well as retinal ganglion cells were strongly stained with their RGNTF contents quantified to be 88%, 100% and 100%, respectively. In the 5-6-day age group, RGNTF contents were significantly reduced to merely 50%, 30% and 80%, respectively.The RGNTF contents reduced further to 0% as age increased to 2 years old. A ~(32)P-DNA probe specific to the first 7 amino acid sequence of RGNTF at its N-terminal end was synthesized and used for in situ hybridization studies. The results revealed that strong hybridized signals (i.e. mRNA of RGNTF) were localized in the same neurons in the superficial layers and deep part of the superior colliculus only in the 0-1-day age grou 相似文献
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螺旋形非对称电视塔风振反应分析 总被引:1,自引:0,他引:1
本文针对存在结构偏心的高耸或高层结构,并结合正在设计的中总高388米、体型为不对称螺肇形的南海明珠电视塔,用取你阶振型并考虑平移扭转耦合振动的计算方法进行了实例计算,并将其计算值与一般计算方法所得值进行比较,最后分析其结果,从而为结构设计提供依据。 相似文献
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