INTRODUCTION, EXPRESSION AND REGENE-RATION OF THE TiT-DNA GENES IN CULTURED CELLS FROM Hyoscyamus muticus +Nicotiana tabacum SOMATIC HYBRIDS

After the cultured cells from Hyoscyamus muticus + Nicotiana tabacum somatic hybridswere cocultivated with different virulent strains of Agrobacterium tumefaciens harboringoctopine- type Ti plasmid or nopaline- type Ti plasmid using the transformation procedurein vitro developed in the present investigation, the TiT- DNA genes were introducedinto the host cells. The onc genes and ocs or nos genes located on TiT- DNA were expres-sed in transformed colonies derived from the cocultivated cells. Although the platingefficiencies of recipient cells were reduced by the agrobacterial treatment, the frequenciesof phytohormone autotrophy ranged from 33.9 to 76 .8% in the cells infected with viru-lent strains in hormone- free conditions, and the frequencies of opine synthase activityamounted to 9.7- 47 .5%. Teratomatous shoots were regenerated from the transformed col-onies. During the course of culture the shoots were no longer to lengthen when theygrew up to 1 -3 cm in length, and they could not be rooted. Follo     

STUDY ON WAYS OF FORCING INTERCELLULAR CYTOPLASM AND CHROMATIN MIGRATION TO TRANSFER EXTERNAL GENETIC SUBSTANCES TO PLANT CELLS BY ARTIFICIAL MOTIVE FORCE

A new cell engineering technique (L. B. technique) was established in our Lab. At first, the physical and chemical methods were used to facilitate the reestablishment of intercellular contacts and plasmodesma channels between different parents, thus forcing cytoplasm and chromatin to pass the cell wall with different qualities and quantities from one cell to others, through the enlarged intercellular plasmodesma channels or the vulnerable regions and the holes on the cell wall formed differently in growth and thickness in the process of cell wall formation to introduce external genetic substances or gene groups into plant cells. There are different ways, frequencies and strengths for the migration between the cells in different growth and development regions or the same growth and development region. In this paper we advance the mechanism of cytoplasm and chromatin migration through the cell wall: There are a large number of plasmodesma channels or vulnerable regions and holes different in growth and th     

6Li的d-α集团结构态的相移分析和广义本征值分析

本文用含有软排斥心势和自旋-轨道非中心势的核子-核(N-N)力和具有d-α集团结构的波函数, 从单道共振群方法(RGM)相移分析和广义本征值分析两方面, 统一研究了⁶Li的(1⁺, 0)基态、(3⁺, 0)第1激发态、、(2⁺, 0)第3激发态、、(1⁺, 0)第5激发态. 结果表明: 从相移分析和相征能量分析两方面都说明了⁶Li的基态主要是l=0的d-α集团结构束缚态, 而第1、3、5激发态主要是l=2的d-α集团结构非束缚态.     

聚丙烯腈热处理的反应机理

本文作者将聚丙烧腈样品分别在200°,250℃……的真空和空气中,以及在180°, 200℃……的氨气中,以逐步升温的方式进行热处理。在每一阶段处理的前后,对样品和产物的红外光谱、重量、它们在浓硫酸和冰醋酸中的溶解度、在浓硫酸中的紫外光谱、以及它们在冰醋酸回流抽提后所余残渣的红外光谱都进行了测量。提出了足以解释所有主要现象的反应机理,其中包括三个重要步骤的反应:(1)特征基团的产生,(2)分子内部的环化,(3)较高温度下的脱氨反应。从每个步骤反应的分析可以看出,热处理所得产物只能是含多核呲啶骈环不长、结构杂乱的高聚物。由于过去学者提出的反应机理只是根据部分实验结果考虑了分子内部环化的发生,而不可能发现到环化的必然中断,因此有些作者根据这机理所作关于产物的半导体电磁性能的解释是值得进一步研究的。     

带约束不可微凸规划的一个次梯度可行方向法

本文提出了一种计算带约束不可微凸规划问题的算法。这是一种利用有关函数的次梯度的可行方向法,它也可以作为[2]中给出的无约束bundle方法在带有不可微凸的约束情形下的推广。本文给出了算法收敛性的证明。对于求解本算法中所用到的计算多面体凸锥与凸多面体间最短距离这个子问题,也给出了一个收敛性得以保证的方法。     

非弹性散射中的四极-八极双声子激发

本文用DWBA方法对两种入射能量E_α=31和和43MeV的⁶⁴Zn(α, α′)⁶⁴Zn*非弹性散射中可能的四极-八极双声子激发进行了研究. 考虑了核力势和库仑势, 零级近似核力势取为Woods-Saxon势, 由靶核振动引起的核力势的非球对称部分V₁作为微扰. 在计算中, V₁取到核表面集体坐标α_λν的二级项, 总初态波函数Ψ⁽⁺⁾_i取到V₁的一级项,目在扭曲波格林函数中略去了作为中间态的吸收道. 此外在双声子激发机制中, 假设直接双声子激发的贡献是主要的, 相继双声子激发的贡献是次要的. 所得的理论角分布与实验符合得相当好. 由这样符合可推知⁶⁴Zn的3.72和4.19MeV能级是四极-八极双声子激发能级, 其角动量和宇称分别为3^－和5^－.     

稀土硝酸盐二甲亚砜络合物的振动光谱研究

合成了十四个稀土硝酸盐二甲亚砜络合物, 并对其振动光谱进行了系统的研究。对络合物的谱带进行了归属和讨论。对重镧系络合物中S=O伸展振动和Ln—O伸展振动的分裂归因于相同基团间的共振偶合。在络合物中, NO_3基组频1775 cm~(-1)因所属群发生变化而产生了分裂, 并可以此来确定NO_3基在络合物中的配位方式。指认了络合物中Ln—O键伸展振动频率, 其随稀土角动量的变化呈现“斜W”效应。     

竞赛不等式的创新证法——向量内积法

文[1]利用匹配因子的方法，构造均值不等式来证明竞赛题中对称和轮换对称不等式．但笔者认为这种匹配因子的方法需要较高的技巧，在实际操作中很难掌握．     

1.32μm泵浦下多模光纤受激喇曼散射

本文报道在1.32μm声光Q开关Nd:YAG激光器泵浦下,从多模光纤中获得由受激喇曼散射及四光子混频产生的宽带非线性散射谱的实验。结果表明,在1.32μm泵浦下产生了明显的连续谱本底,并认为它是由光纤零材料色散区的宽带参量增益四光子混频产生的。观察到激光器1.34μm微弱输出在光纤中明显放大的新现象,并指出放大来自玻璃光纤的宽带喇曼增益。     

STUDIES ON THE ORIGIN OF B CHROMOSOMES IN RYE

The morphological structure, classification and meiotic behaviour as well as the possible origin of the extra centric fragment produced after intercellular chromatin migration (cytomixis) in pollen mother cells during synizesis in rye have been studied by the squash and Giemsa·C-banding techniques. The results showed that the structures of extra centric fragments are metacentric, submetacentric, telocentric or subtelocentric, being quite different from each other. Their originations are stochastic, because any chromosome can be formed by the fragment. The meiotic behaviour of extra centric fragments is identical with that of B chromosomes. We consider that extra centric fragments are B chromosomes. The fragments originated from the fragmentation of A chromosomes at synizesis during cytomixis. Thus we consider that cytomixis could be one of the origins of B chromosomes.