In vitro evaluation of cytotoxic and inflammatory properties of silica nanoparticles of different sizes in murine RAW 264.7 macrophages

The biological response to four well-characterized amorphous silica nanoparticles was investigated in RAW 264.7 macrophages in view of their potential application as drug carriers to sites of inflammation. All silica nanoparticles-induced cell membrane damage, reduced metabolic activity, generated ROS and released various cytokines, but to different extents. Two silica nanoparticles of 34 nm (A and B) with different zetapotentials were more cytotoxic than (aggregated) 11 and 248 nm nanoparticles, while cytokines were mostly induced by the (aggregated) 11 nm and only one of the 34 nm nanoparticles (34A). The results indicate that specific silica nanoparticles may have counterproductive effects, for example when used as carriers of anti-inflammatory drugs. The physicochemical properties determining the response of nanoparticles vary for different responses, implying that a screening approach for the safe development of nanoparticles needs to consider the role of combinations of (dynamic) physicochemical properties and needs to include multiple toxicity endpoints.     

Possibilities and limitations of synchrotron X-ray powder diffraction with double crystal and double multilayer monochromators for microscopic speciation studies

The performance of a combined microbeam X-ray fluorescence/X-ray powder diffraction (XRF/XRPD) measurement station at Hamburger Synchrotronstrahlungslabor (HASYLAB) Beamline L is discussed in comparison to that at European Synchrotron Radiation Facility (ESRF) ID18F/ID22. The angular resolution in the X-ray diffractograms is documented when different combinations of X-ray source, optics and X-ray diffraction detectors are employed. Typical angular resolution values in the range 0.3–0.5° are obtained at the bending magnet source when a ‘pink’ beam form of excitation is employed. A similar setup at European Synchrotron Radiation Facility beamlines ID18F and ID22 allows to reach angular resolution values of 0.1–0.15°. In order to document the possibilities and limitations for speciation of metals in environmental materials by means of Hamburger Synchrotronstrahlungslabor Beamline L X-ray fluorescence/X-ray powder diffraction setup, two case studies are discussed, one involved in the identification of the crystal phases in which heavy metals such as chromium, iron, barium and lead are present in polluted soils of an industrial site (Val Basento, Italy) and another involved in the speciation of uranium in depleted uranium particles (Ceja Mountains, Kosovo). In the former case, the angular resolution is sufficient to allow identification of most crystalline phases present while in the latter case, it is necessary to dispose of an angular resolution of ca. 0.2° to distinguish between different forms of oxidized uranium.     

An upper bound on the cycle time of a stochastic marked graph using incomplete information on the transition firing time distributions

Stochastic marked graphs, a special class of stochastic timed Petri nets, are used for modelling and analyzing decision-free dynamic systems with uncertainties in timing. The model allows evaluating the performance of such systems under a cyclic process. Given the probabilistic characteristics of the transition times, the cycle time of the system can be determined from the initial marking. In this contribution, we compute an upper bound on the cycle time of a stochastic marked graph in case the probabilistic characteristics of the transition times are not fully specified.     

Spatially resolved micro-X-ray fluorescence and micro-X-ray absorption fine structure study of a fractured granite bore core following a radiotracer experiment

Spatially resolved X-ray absorption and fluorescence investigation with a micrometer-scale resolution on actinide-containing samples provide information necessary for safety assessment of nuclear waste disposal. In this paper one example of such an experiment is presented. This example entails neptunium speciation in a fractured granite bore core from the Swedish Äspö Hard Rock Laboratory following a radiotracer experiment using µ-XAFS and µ-XRF. In order to probe micro-volumes below the surface in the granite samples and thereby avoid potential changes in the Np speciation during cutting of the bore core, a confocal irradiation–detection geometry is employed. µ-XAFS results for a selected granite bore core cross section with ~ 3 nmol Np/g reveal that Np, originally introduced as Np(V) in the tracer cocktail, is present in the granite in its reduced Np(IV) form. The Np(IV) is often present as particles, tens of µm in size. Elemental distribution maps show the tracer Np to be located in fissures and permeable channels not larger than 100 µm. The Np distribution appears often correlated with Zn also present in some fissures. We observe small granite fissures containing Fe (presumably Fe(II)), where we do not detect any Np. It is feasible that inflowing Np(V) has a shorter residence time in large fractures, while in the smaller fissures migration is slower, leading to longer residence times, i.e., reaction times, where it is reduced to less soluble Np(IV) and becomes thereby immobilized.     

A liquid chromatography – tandem mass spectrometry method to measure a selected panel of uremic retention solutes derived from endogenous and colonic microbial metabolism

Chronic kidney disease (CKD) is associated with an increased risk of mortality and cardiovascular disease, which is, at least partly, mediated by the accumulation of so-called uremic retention solutes. Although there has been an increasing interest in the behavior of these solutes, derived from both the endogenous and colonic microbial metabolism, methods to simultaneously and accurately measure a broad panel of relevant uremic retention solutes remain scarce.     

Wide tuning of silicon-on-insulator ring resonators with a liquid crystal cladding

Wide electrical tuning of silicon-on-insulator ring resonators is demonstrated using a top cladding layer of nematic liquid crystals. A tuning range of 31?nm is demonstrated for ring resonators guiding the TM mode, covering nearly the entire C-band of optical communications. Ring resonators guiding the TE mode can be tuned over 4.5?nm. The combination of a liquid crystal director calculation and a fully anisotropic mode solver confirms the interpretation of these experimental results. The realization of broad and low-power tuning in silicon-on-insulator opens up new opportunities in the field of tunable lasers, filters, and detectors.     

µ-X-ray fluorescence and µ-X-ray diffraction investigations of sediment from the Ruprechtov nuclear waste disposal natural analog site

Results from combined µ-XRF and µ-XRD investigations of a uranium-rich tertiary sediment from a nuclear repository natural analog site in Ruprechtov, Czech Republic, are reported. The goal of the investigations is to identify arsenopyrite from diffraction patterns obtained in As-rich areas of the sample. Arsenopyrite has been revealed to be present in the sediment as thin coatings on the surface of Fe nodules from previous µ-XANES results and observed linear dependencies between Fe and As(0) distributions obtained from µ-XRF measurements. In thin sections of the sediment investigated here we find, however, neither diffraction reflections expected for arsenopyrite nor for other As-sulfides. The As(0) phase is likely present in the form of very small grains, or it is amorphous. Statistical analysis of the µ-XRD data using unsupervised classification and principle component analysis from selected sample areas also indicate that pyrite and siderite, but no arsenopyrite, are the sole diffracting phases. That pyrite and siderite are found in the same sample is an indication that the pH of the sediment environment during diagenesis must have been neutral. This allows us to refine our hypothesis for the mechanism of uranium immobilization.     

X-ray Diffraction Mapping for Cultural Heritage Science: a Review of Experimental Configurations and Applications

X-ray diffraction (XRD) mapping consists in the acquisition of XRD patterns at each pixel (or voxel) of an area (or volume). The spatial resolution ranges from the micrometer (μXRD) to the millimeter (MA-XRD) scale, making the technique relevant for tiny samples up to large objects. Although XRD is primarily used for the identification of different materials in (complex) mixtures, additional information regarding the crystallite size, their orientation, and their in-depth distribution can also be obtained. Through mapping, these different types of information can be located on the studied sample/object. Cultural heritage objects are usually highly heterogeneous, and contain both original and later (degradation, conservation) materials. Their structural characterization is required both to determine ancient manufacturing processes and to evaluate their conservation state. Together with other mapping techniques, XRD mapping is increasingly used for these purposes. Here, the authors review applications as well as the various configurations for XRD mapping (synchrotron/laboratory X-ray source, poly-/monochromatic beam, micro/macro beam, 2D/3D, transmission/reflection mode). On-going hardware and software developments will further establish the technique as a key tool in heritage science.