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1.
In a previous paper the authors, together with Balwant Singh, introduced an elementwisc characterization of weak subintegrality for an extension A?B of commutative rings. In the present paper we improve our methods for working with this characterization. This leads us to propose two new types of “elementary weakly subintegral extension”. 相似文献
2.
Sarah Fitzpatrick Les Ebdon Michael E. Foulkes 《International journal of environmental analytical chemistry》2013,93(11-12):835-841
A method is presented for arsenic speciation analysis of an oyster sample using ion chromatography coupled with an inductively coupled plasma mass spectrometry (ICP-MS) instrument. A strong anion exchange resin was employed with a step gradient elution of 0.1 mM/0.1 M K 2 SO 4 at pH 10.2. Arsenobetaine and dimethylarsinic acid were determined following extraction based on trypsin enzymolysis with 95-100% extraction efficiency. Limits of detection in the range 0.1-0.3 mg kg m 1 of arsenic were obtained for organic arsenic species. No inorganic arsenic was detected. Validation was performed using TORT-2 as a certified reference material. Although high performance liquid chromatography (HPLC) coupled to ICP-MS is an effective method for speciation analysis it is not always necessary to obtain such a detailed picture. A simple liquid chromatographic separation technique based upon mini-column technology is presented. It was developed to obtain a fast, efficient and reliable separation of inorganic from organic, i.e. assumed toxic from non-toxic, arsenic and selenium species suitable for use as an initial screening method for environmental analysis. Two types of strong anion exchange resin were tested. Excellent separation was obtained for both min-column resins and analysis times were within 7 min. Limits of detection obtained for inorganic arsenic, organic arsenic, selenomethionine, Se IV and Se VI were 1.6, 1.8, 66, 32 and 22 µg kg m 1 , respectively. 相似文献
3.
Wei-Gang Hu Scott Jager Damon Chau Dave Mah Les P. Nagata 《Applied biochemistry and biotechnology》2010,160(4):1206-1216
In order to develop a recombinant full-length human anti-botulinum neurotoxin A (BoNT/A) antibody, human peripheral blood
mononuclear cells (PBMC) were collected from three healthy volunteers and induced for BoNT/A-specific immune response by in
vitro immunization. The genes encoding human Fd fragment, consisting of antibody heavy chain variable region and constant
region 1 with the genes encoding antibody light chain, were cloned from the immunized PBMC. Afterwards, one combinatory human
antigen-binding fragment (Fab) library was constructed using a lambda phage vector system. The size of the constructed library
was approximately 105
Escherichia coli transformants. After screening the library by BoNT/A antigen using a plaque lifting with immunostaining approach, 55 clones
were identified as positive. The Fab gene of the most reactive clone exhibiting particularly strong BoNT/A binding signal
was further subcloned into a full-length human IgG1 antibody gene template in an adenoviral expression vector, in which the
heavy and light chains were linked by a foot-and-mouth-disease virus-derived 2A self-cleavage peptide under a single promoter.
After the full-length human IgG1 was expressed in mammalian cells and purified with protein L column, sodium dodecyl sulfate-polyacrylamide
gel electrophoresis showed that the heavy and light chains of the antibody were cleaved completely. The affinity expressed
as the dissociation constant (K
d) for the recombinant human antibody to bind to BoNT/A was determined by indirect enzyme-linked immunosorbent assay and results
confirmed that the recombinant full-length human antibody retained BoNT/A-binding specificity with K
d value of 10−7 M. 相似文献
4.
5.
Three new lacunar-type derivatives of β-unsubstituted dibenzotetraaza[14]annulene have been synthesized along with a range of their open-chain pendant group containing counterparts. The crystal structures of two representative products have been determined and the noncovalent interactions explored. A number of C-H?π and π?π interactions involving aliphatic chains and aromatic regions of the macrocycle have been evidenced. The relative ease and high yield of lacunization are suggested to be largely due to self-assembly processes, driven by favorable noncovalent interactions between reacting units. 相似文献
6.
7.
P. Hlubina D. Ciprian J. Luňáček M. Lesňák 《Applied physics. B, Lasers and optics》2006,84(3):511-516
We present a white-light spectral interferometric technique for measuring the thickness of SiO2 thin film on a silicon wafer. The technique utilizes a slightly dispersive Michelson interferometer with a cube beam splitter and a fibre-optic spectrometer to record channelled spectra in two configurations. In the first, a standard configuration with two identical metallic mirrors, the recorded channelled spectrum is fitted to the theoretical one to determine the effective thickness of the beam splitter made of BK7 optical glass. In the second configuration one of the mirrors is replaced by SiO2 thin film on the silicon wafer and the recorded channelled spectrum is fitted to the theoretical one to determine the thin-film thickness. We consider multiple reflection within the thin-film structure, use the optical constants for all the materials involved in the set-up, and confirm very good agreement between theory and experiment. The technique is applied to four samples with various SiO2 film thicknesses. PACS 07.60.Ly; 68.55.Jk; 78.20 相似文献
8.
9.
The effect of sodium interference on the analytical performance of inductively-coupled plasma/atomic emission spectrometry is evaluated. A suitable response function based on the square of the percentage interference was used for multi-element simplex optimization to locate a minimum interference zone in the argon plasma. The plasma was optimized for minimum easily ionizable element interference for Tl, Mn, Mg and Sr. Interferences could be removed completely at the cost of losing sensitivity by up to a factor of 4. The optimization correctly identified an interference-free zone in the plasma. Stability of the injector gas flow rate was found to be vital for accurate location and long-term stability of this zone. Pressure fluctuations during nebulization were shown to be the major cause of high-frequency fluctuations of this zone. 相似文献
10.
Journal of the Operational Research Society - 相似文献