Inactivation of bovine immunodeficiency virus photodynamic therapy with HMME

To investigate the effect of photodynamic therapy （PDT） with hematoporphrin monomethyl ether （HMME） on bovine immunodeficiency virus （BIV） can provide the basis theory for photoinactivation of human immunodeficiency virus （HIV）. To assess the protection of HMME-PDT on the cell line Cf2Th infected with BIVR29 by 3-（4,5）-dimethylthiahiazol-2-yl-3,5-di-phenytetrazolium bromide （MTT） with power density of 5 and 25 mW/cm^2 and energy density from 0.6 to 3 J/cm^2. To observe the inhibition of membrane fusion using a new reporter cell line BIVE by fluorescence microscope. HMME-PDT has significant protectant effects on Cf2Th-BIVR29 with both power densities, especially in the group of high power density. Fluorescent microscope shows that there is no significant difference between the group of PDT and control, which means PDT could not inhibit the BIV-mediated membrane fusion.     

酒精处理后鼠脑内单胺氧化酶活性及单胺神经递质代谢变化的测定

应用高效液相色谱法测定了慢性酒精处理后胎鼠脑中单胺氧化酶(MAO)的活性,以及多巴胺(DA)、5-羟色胺(5-HT)和它们的代谢产物的含量。在怀孕的最后一周内对SD母鼠灌胃酒精,对照组用生理盐水处理,分娩后取胎鼠脑组织进行检测。胎鼠脑线粒体MAO的活性随酒精染毒剂量的增加而递减,呈现出剂量反应关系;与盐水对照组相比,酒精暴露可改变胎鼠全脑中DA和5-HT的水平,但统计结果无显著性差异。其中,HVA和5-HIAA的含量在4.0g/kg酒精处理后显著减少(P0.05)。慢性酒精暴露后MAO活性的下降及DA、5-HT的代谢变化可能在中枢神经系统的发育障碍中发挥了重要作用。     

类星体的极软X过剩

在强软X类星体样品中,我们根据用Einsten天文台IPC决定的N_H与银河21cm线决定的N_H值的关系,构成极软X过剩类星体样品,并导出在0.3keV和0.5keV之间的X谱指数α_E。α_E明显大于0.5—3.5keV间的谱指数α_E。这表明0.3—0.5keV正是类星体紫外到X段大包右侧的急剧下降部分。这些结果将有助于导出这类类星体紫外到软X大包的组合模型。