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Alipov A. A. Lekontseva N. V. Mikhailina A. O. Fando M. S. Tishchenko S. V. Nikulin A. D. 《Crystallography Reports》2021,66(5):791-796
Crystallography Reports - The bacterial protein Hfq is a member of a large family of Lsm proteins, which are characterized by a conserved tertiary structure and form a stable ring-shaped quaternary... 相似文献
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Lopez-Canovas L Bravo L Herrera J Riveron AM Javer E Sanchez A Fando R Noa MD Fernandez A 《Electrophoresis》2006,27(14):2857-2864
DNA molecules of Vibrio cholerae and Aeromonas species were prepared by incubating immobilized cells for 4 and 2 h, respectively, with a nonenzymatic solution that contains chemical reagents only (NDSUPlus). This method gave results as reproducible as the enzymatic one that uses proteinase K, and rendered DNA molecules suitable for fingerprinting by mini-CHEF electrophoresis. As rapid DNA separations at high electric field are achieved in mini-CHEF chamber with low heat evolution, DNA restriction fragments were separated in 5 h at 10 V/cm in a single resolution window. Then, fragment separations in three resolution windows were done in 15 h. This time is shorter than the one needed by the large CHEF chamber for resolving fragments in a single resolution window. Three windows permitted to include larger numbers of restriction fragments in the calculation of isolate similarities. Both sample preparation and mini-CHEF electrophoresis may represent an alternative for performing massive epidemiological studies of V. cholerae and Aeromonas species. 相似文献
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