大豆原生质体经体细胞胚再生植株

从栽培大豆(Glycine max L)的未成熟子叶游离的原生质体,以Gelrite bead法包埋,培养在大豆根瘤产物(天冬酰胺、谷氨酰胺、尿囊素、尿囊酸等)为主要氮源的ZSP培养基中,形成了胚性愈伤组织。该愈伤组织在体细胞胚分化培养基上直接分化出体细胞胚,并进一步诱导出再生植株,进入正常发育而开花。     

Plant Regeneration From Soybean (Glycine max L.) Protoplasts via Somatic Embryogenesis

Protoplasts isolated from immature cotyledons of cultivated soybean (Glycine max L.) were cultured by Gelrite bead method in ZSP medium with soybean root nodule products (asparagine, glutamine, allantoin, allantoic acid, etc.) as the main nitrogen resources. Embryogenic calli were initiated and transferred to somatic embryo differentiation medium. Somatic embryos were formed from embryogenic calli and converted to plants. The regenerated plants normally grew and flowered.