ESTABLISHMENT and CHARACTERIZATION OF A MONOCLONAL ANTIBODY RECOGNIZING THE DEWAR ISOMERS OF(6–4)PHOTOPRODUCTS

Abstract— We established a monoclonal antibody(DEM–1) that recognizes UV-induced DNA damage other than cyclobutane pyrimidine dimers or(6–4)photoproducts. The binding ofDEM–1 antibody to 254 nm UV-irradiated DNA increased with subsequent exposure to UV wavelengths longer than 310 nm, whereas that of the 64M-2 antibody specific for the(6–4)photoproduct decreased with this treatment. Furthermore, the increase inDEM–1 binding was inhibited by the presence of the 64M-2 antibody during the exposure. We concluded that theDEM–1 antibody specifically recognized the Dewar photoproduct, which is the isomeric form of the(6–4)photoproduct. TheDEM–1 antibody, however, also bound to DNA irradiated with high fluences of 254 nm UV, suggesting that 254 nm UV could induce Dewar photoproducts without subsequent exposure to longer wavelengths of UV. Furthermore, an action spectral study demonstrated that 254 nm was the most efficient wavelength for Dewar photoproduct induction in the region from 254 to 365 nm, as well as cyclobutane dimers and(6–4)photoproducts, although the action spectrum values in the U V-B region were significantly higher compared with those for cyclobutane dimer and(6–4)photoproduct induction.     

Magnetoresonant softening of solids

The main goal of this work was to detect the magnetosensitive spin-dependent reactions between structural defects and their influence on the plasticity of ionic crystals. It was found that coincidence of quantum energy of a microwave field with the Zeeman splitting energy of electronic spin sublevels of paramagnetic centres leads to an increase in the relaxation rate of assemblages of the defects excited by quenching and, as a result, to resonant weakening of crystals. It means that the electron and nuclear spin states of the defects are of importance in the formation of plasticity in crystals.     

Transcutaneous application of carbon dioxide improves contractures after immobilization of rat knee joint

Objective: Joint contractures are a major complication following joint immobilization. However, no fully effective treatment has yet been found. Recently, carbon dioxide (CO₂) therapy was developed and verified this therapeutic application in various disorders. We aimed to verify the efficacy of transcutaneous CO₂ therapy for immobilization-induced joint contracture. Method: Twenty-two Wistar rats were randomly assigned to three groups: caged control, those untreated after joint immobilization, and those treated after joint immobilization. The rats were treated with CO₂ for 20 min once a daily either during immobilization, (prevention) or during remobilization after immobilization (treatment). Knee extension motion was measured with a goniometer, and the muscular and articular factors responsible for contractures were calculated. We evaluated muscle fibrosis, fibrosis-related genes (collagen Type 1α1 and TGF-β1) in muscles, synovial intima''s length, and fibrosis-related proteins (Type I collagen and TGF-β1) in the joint capsules. Results: CO₂ therapy for prevention and treatment improved the knee extension motion. Muscular and articular factors decreased in rats of the treatment group. The muscular fibrosis of treated rats decreased in the treatment group. Although CO₂ therapy did not repress the increased expression of collagen Type 1α1, the therapy decreased the expression of TGF-β1 in the treatment group. CO₂ therapy for treatment improved the shortening of the synovial membrane after immobilization and decreased the immunolabeling of TGF-β1 in the joint capsules. Conclusions: CO₂ therapy may prevent and treat contractures after joint immobilization, and appears to be more effective as a treatment strategy for the deterioration of contractures during remobilization.     

Spin relaxation in acyl radicals measured using spin correlated radical pair (SCRP) polarization in flexible biradicals

Time resolved electron paramagnetic resonance spectra and the decay kinetics of spin correlated radical pair (SCRP) polarization in an acyl-benzyl biradical were measured over a wide temperature range (180–274 K). The major mechanism of intersystem crossing in this biradical is the spin rotation induced relaxation of the acyl moiety, which is associated with the rotation of the carbonyl group about the neighbouring CC bond axis. This relaxation determines the decay rate of the polarization. The relaxation time is largely viscosity independent; it changes by a factor of less than two going from room temperature (60 ns) to 180 K (110 ns) in 2-propanol.     

MULTITRANSITIVITY OF CALOGERO-MOSER SPACES

Let G be the group of unimodular automorphisms of a free associative ?-algebra on two generators. A theorem of G. Wilson and the first author [BW] asserts that the natural action of G on the Calogero-Moser spaces C _n is transitive for all n ? ?. We extend this result in two ways: first, we prove that the action of G on C _n is doubly transitive, meaning that G acts transitively on the configuration space of ordered pairs of distinct points in C _n ; second, we prove that the diagonal action of G on \( {C}_{n_1}\times {C}_{n_2}\times \cdots \times {C}_{n_m} \) is transitive provided n ₁,?n ₂,?…,?n _m are pairwise distinct numbers.     

𝔾a-ACTIONS ON AFFINE CONES

An affine algebraic variety X is called cylindrical if it contains a principal Zariski dense open cylinder U ? Z × A1. A polarized projective variety (Y, H) is called cylindrical if it contains a cylinder U = Y \ supp D, where D is an effective Q-divisor on Y such that [D] ∈ Q+[H] in PicQ(Y ). We show that cylindricity of a polarized projective variety is equivalent to that of a certain Veronese affine cone over this variety. This gives a criterion of the existence of a unipotent group action on an affine cone.     

Quantitative Siegel's theorem for Galois coverings

It is known that Siegels theorem on integral points is effective for Galoiscoverings of the projective line. In this paper we obtain a quantitative version of this result, giving an explicit upper bound for the heights of S-integral K-rational points in terms of the number field K, the set of places S and the defining equation of the curve.Our main tools are Bakers theory of linear forms in logarithms and thequantitative Eisenstein theorem due to Schmidt, Dwork and van der Poorten.