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The kinetics of peptide release during in vitro digestion of 4 protein sources (casein, cod protein, soy protein, and gluten) were investigated. Samples were sequentially hydrolyzed with pepsin (30 min) and pancreatin (2, 4, or 6 h) in a dialysis cell with continuous removal of digestion products. Nondialyzed digests were fractionated by ion-exchange chromatography and ultrafiltration. Animal proteins were digested at a greater rate than plant proteins. Target amino acids of specific enzymes appeared more rapidly in the dialyzed fractions when compared to other amino acids. Throughout the hydrolysis, nondialyzed digests contained a higher proportion of peptide mixtures with basic-neutral properties. Except for gluten, peptide fractions with molecular weights that exceeded 10 kDa (basic-neutral, BN > 10) were rapidly hydrolyzed during the first 2 h of pancreatin digestion. The kinetics of release and the composition of peptide fractions were different when the protein sources were compared. The analysis of amino acids revealed that threonine and proline proportions were relatively high in BN > 10 and in peptide fractions with molecular weight between 10-1 kDa (BN 10-1), while tyrosine, phenylalanine, lysine, and arginine predominated in the low molecular weight (<1 kDa) fractions. More resistant peptides were generally rich in proline and glutamic acid. The role of in vitro digestion assays in dietary protein quality evaluation is discussed. 相似文献
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Wenjun Tang Dr. Andrew G. Capacci Xudong Wei Dr. Wenjie Li Dr. Andre White Dr. Nitinchandra D. Patel Jolaine Savoie Joe J. Gao Sonia Rodriguez Dr. Bo Qu Dr. Nizar Haddad Dr. Bruce Z. Lu Dr. Dhileepkumar Krishnamurthy Dr. Nathan K. Yee Dr. Chris H. Senanayake Dr. 《Angewandte Chemie (International ed. in English)》2010,49(34):5879-5883
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We present a rigorous mathematical treatment of the zero-field orbital magnetic susceptibility of a non-interacting Bloch
electron gas, at fixed temperature and density, for both metals and semiconductors/insulators. In particular, we obtain the
Landau-Peierls formula in the low temperature and density limit as conjectured by Kjeldaas and Kohn (Phys Rev 105:806–813,
1957). 相似文献
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Camille Houle Dr. Paul R. Savoie Clotilde Davies Damien Jardel Prof. Pier Alexandre Champagne Dr. Brigitte Bibal Prof. Jean-François Paquin 《Chemistry (Weinheim an der Bergstrasse, Germany)》2020,26(46):10620-10625
We describe the first thiourea-catalyzed C−F bond activation. The use of a thiourea catalyst and Ti(OiPr)4 as a fluoride scavenger allows the amination of benzylic fluorides to proceed in moderate to excellent yields. Preliminary results with S- and O-based nucleophiles are also presented. DFT calculations reveal the importance of hydrogen bonds between the catalyst and the fluorine atom of the substrate to lower the activation energy during the transition state. 相似文献
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Malatesti N Hudson R Smith K Savoie H Rix K Welham K Boyle RW 《Photochemistry and photobiology》2006,82(3):746-749
Two boron complexes of 5-phenyldipyrromethenes bearing isothiocyanate groups on the phenyl ring have been synthesized for the first time. The utility of these new fluorescence probes for labeling biologically relevant proteins is demonstrated on two monoclonal antibodies that bind to antigens overexpressed on cancer cells. Spectral comparison of the two structures reveals significant photophysical differences, including bathochromically shifted excitation and emission bands, increased molar absorptivity and a large increase in fluorescence quantum yield of approximately 10 times. Differences in photophysical parameters are linked to hindered rotation of the phenyl ring in one of the probes. 相似文献
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Belkacemi Khaled Turcotte Ginette Halleux Damien de Savoie Philippe 《Applied biochemistry and biotechnology》1998,(1):441-462
Lignocellulosic materials derived from forages, namely timothy grass, alfalfa, reed canary grass, and agricultural residues,
such as corn stalks and barley straw, were pretreated using ammonia fiber explosion (AFEX) process. The pretreated materials
were directly saccharified by cellulolytic enzymes. Sixty to 80% of theoretical yield of sugars were obtained from the pretreated
biomasses. Subsequent ethanolic fermentation of the hydrolysates byPachysolen tannophilus ATCC 32691 resulted in 40-60% of theoretical yield after 24 h, based on the sugars present in the hydrolysates. The uptake
of sugars was not complete, indicating a possible inhibitory effect onP. tannophilus during the fermentation of these substrates. 相似文献
7.
Raman spectroscopic measurements on aqueous solutions of poly(dG) · poly(dC)indicate that the conformation of the polynucleotides in this double helicalcomplex are distributed between the A and B types at room temperature, the Aform being predominant at –15°C and decreasing progressively upon raising thetemperature to 65°C. A reversible pretransition has been found in this complexnear 70°C. Modifications in the spectra at this temperature indicate no majorconformational changes, but rather suggest altered base pairing and hydration ofthe carbonyl groups, accompanied by a slight distortion of the double helix,resulting in a slightly reduced stacking of the cytosine bases. Measurements inself-pressurized solutions of the complex at high temperature show that it meltsat 103°C in 0.1M NaCl solution (107°C in 0.5M NaCl). These values are somewhatlower than those we have determined in the same manner for the complexpoly(dG-dC) · poly(dG-dC): 117°C in 0.1M MgCl2 and 113°C or higher in 0.1MNaCl solution. 相似文献
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Inside Cover: Organic Photovoltaics: Elucidating the Ultra‐Fast Exciton Dissociation Mechanism in Disordered Materials (Angew. Chem. Int. Ed. 29/2014) 下载免费PDF全文