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排序方式: 共有127条查询结果,搜索用时 31 毫秒
1.
Dr. Muhammad Ehsan Dr. Yang Du Jonas S. Mortensen Dr. Parameswaran Hariharan Qianhui Qu Lubna Ghani Dr. Manabendra Das Anne Grethen Prof. Bernadette Byrne Prof. Georgios Skiniotis Prof. Sandro Keller Prof. Claus J. Loland Prof. Lan Guan Prof. Brian K. Kobilka Prof. Pil Seok Chae 《Chemistry (Weinheim an der Bergstrasse, Germany)》2019,25(49):11545-11554
Amphipathic agents are widely used in various fields including biomedical sciences. Micelle-forming detergents are particularly useful for in vitro membrane-protein characterization. As many conventional detergents are limited in their ability to stabilize membrane proteins, it is necessary to develop novel detergents to facilitate membrane-protein research. In the current study, we developed novel trimaltoside detergents with an alkyl pendant-bearing terphenyl unit as a hydrophobic group, designated terphenyl-cored maltosides (TPMs). We found that the geometry of the detergent hydrophobic group substantially impacts detergent self-assembly behavior, as well as detergent efficacy for membrane-protein stabilization. TPM-Vs, with a bent terphenyl group, were superior to the linear counterparts (TPM-Ls) at stabilizing multiple membrane proteins. The favorable protein stabilization efficacy of these bent TPMs is likely associated with a binding mode with membrane proteins distinct from conventional detergents and facial amphiphiles. When compared to n-dodecyl-β-d -maltoside (DDM), most TPMs were superior or comparable to this gold standard detergent at stabilizing membrane proteins. Notably, TPM-L3 was particularly effective at stabilizing the human β2 adrenergic receptor (β2AR), a G-protein coupled receptor, and its complex with Gs protein. Thus, the current study not only provides novel detergent tools that are useful for membrane-protein study, but also suggests a critical role for detergent hydrophobic group geometry in governing detergent efficacy. 相似文献
2.
Milad Ghani Zahra Jafari Behrooz Maleki Maryam Chamani 《Journal of separation science》2023,46(2):2200745
Herein, polydopamine-coated Fe3O4 spheres were synthesized using a very simple, easy, cost-effective, efficient, and fast method. First, magnetic nanoparticles (Fe3O4) were synthesized and were followed by accommodating polydopamine on the surface of the prepared Fe3O4. The prepared polydopamine-coated Fe3O4 spheres were utilized as a sorbent in magnetic solid phase extraction of gemfibrozil and warfarin (as the model analytes). The extracted model analytes were desorbed by a suitable organic solvent and were analyzed by high-performance liquid chromatography. Under optimized condition, the linearity of the method was in the range of 0.1–200.0 μg/L for the selected analytes in water. The limits of detection were calculated to be in the range of 0.026–0.055 μg/L for warfarin and gemfibrozil, respectively. The limits of quantification were calculated to be in the range of 0.089–0.185 μg/L. The inter-day and intra-day relative standard deviations were determined to be in the range of 1.4%–3.3% in three concentrations in order to calculate the method precision. Furthermore, the enrichment factors were found to be 78 and 81 for warfarin and gemfibrozil, respectively. Moreover, the calculated absolute recoveries were between 78% and 81%. The obtained recoveries indicated that the method was useful and applicable in complicated real samples. 相似文献
3.
Isa Illyas Md Sharif Sharifah Norain Mohd Hashim Norhayati Ghani Sulaiman Ab 《Ionics》2015,21(10):2949-2958
Ionics - A mercury(II) sensor was developed by using single-walled carbon nanotube (SWCNT) paste electrode modified with layered double Zn/Al hydroxide-3(4-methoxyphenyl)propionate nanocomposite... 相似文献
4.
5.
Nabil N. AL‐Hashimi Rand O. Shahin Aqeel N. AL‐Hashimi Ajeal M. Al Ajeal Lubna H. Tahtamouni Chanbasha Basheer 《Biomedical chromatography : BMC》2019,33(2)
A new cetyl‐alcohol‐reinforced hollow fiber solid/liquid‐phase microextraction (CA–HF–SLPME) followed by high‐performance liquid chromatography–diode array detection (HPLC–DAD) method was developed for simultaneous determination of ezetimibe and simvastatin in human plasma and urine samples. To prepare the CA–HF–SLPME device, the cetyl‐alcohol was immobilized into the pores of a 2.5 cm hollow fiber micro‐tube and the lumen of the micro‐tube was filled with 1‐octanol with the two ends sealed. Afterwards, the prepared device was introduced into 10 mL of the sample solution containing the analytes with agitation. Under optimized conditions, calibration curves plotted in spiked plasma and urine samples were linear in the ranges of 0.363–25/0.49–25 μg L?1 for ezetimibe/simvastatin and 0.193–25/0.312–25 μg L?1 for ezetimibe/simvastatin in plasma and urine samples, respectively. The limit of detection was 0.109/0.174 μg L?1 for ezetimibe/simvastatin in plasma and 0.058/0.093 μg L?1 for ezetimibe/simvastatin in urine. As a potential application, the proposed method was applied to determine the concentration of selected analytes in patient plasma and urine samples after medication and satisfactory results were achieved. In comparison with reference methods, the CA–HF–SLPME–HPLC–DAD method demonstrates considerable potential in the biopharmaceutical analysis of selected drugs. 相似文献
6.
Anish Khan Aftab Aslam Parwaz Khan Abdullah M. Asiri Malik Abdul Rub Mohammed M. Rahman Sulaiman Ab. Ghani 《Mikrochimica acta》2014,181(9-10):1049-1057
A composite was prepared from copper and graphene oxide (Cu-GO) by in-situ chemical reduction of a mixture containing GO and Cu(II) ions with potassium borohydride. The morphology and structure of the composite were confirmed by various physicochemical techniques. The materials were used in a tyrosinase-based microbiosensor where the enzyme is immobilized in a biocompatible matrix consisting of poly(ortho-phenylene diamine) and Cu-GO. The composite was deposited on the surface of an 8-μm thick carbon fiber microelectrode. The role of each component in the sensing layer was systematically investigated with respect to the analytical performance of the system. In its optimal configuration, the biosensor demonstrated (a) a sensitivity of 6.1?±?3 nA mM-1 dopamine (DA), (b) a linear response to DA (with a Michaelis-Menten constant of 0.29?±?0.03 mM), (c) good selectivity over ascorbic acid and uric acid, and (d) a high blocking capacity (112.2?±?2 mM) for ascorbic acid. Figure
Poly(o-phenylenediamine) electropolymerized carbon fiber electrode with sensitivity towards dopamine (DA) is 6.1?±?3 nA mM?1 supported by Cu-GO. The linear range for DA is 0.29?±?0.03 mM with 0.033 μM LOD and fast response time of <8 s with ascorbic acid blocking capacity of 112.2?±?2 mM AA. Studies on different ratio of Glu/Tyr revealed that 10:3 gave best overall response. 相似文献
7.
A. A. Latif H. Ahmad N. A. Awang M. Z. Zulkifli C. H. Pua Z. A. Ghani S. W. Harun 《Laser Physics》2011,21(4):712-717
In this paper, a design of a High Power Tunable Fiber Laser (HP-TFL) in C-band region from 1536.7 to 1548.6 nm is set forth
with Erbium Doped Fibers (EDFs) being used as a seeding signal and a booster amplifier. With a 1 × 16 channels Arrayed Waveguide
Grating (AWG), this setup is capable of generating 16 different wavelengths with an average output power of 20.7 dBm. 相似文献
8.
Nusrat Lubna Gregory AunerRahul Patwa Hans HerfurthGolam Newaz 《Applied Surface Science》2011,257(10):4749-4753
Thin film materials are widely used in the fabrication of semiconductor microelectronic devices. In thin film deposition, cleanliness of substrate surface have become critically important as over 50% of yield losses in integrated circuit fabrication are caused by microcontamination [1]. There are many wafer cleaning techniques. The most successful approach for silicon wafer cleaning technique is RCA clean [2]. But for glass substrate it is still not known which procedure of cleaning is the best. This paper provides an understanding of the right way of glass wafer cleaning method, with a focus towards identifying good bond strength. Two wafer cleaning techniques have been used for cleaning glass substrates in the context of laser micro-joining of dissimilar substrates. First cleaning procedure involves two steps, first cleaning in acetone solution and then in DI water solution. After each step dried with N2. Second cleaning procedure involves four steps, first cleaning with 1% Alconox solution, second in DI water, third in acetone solution and finally in a methanol solution and dried with N2 after each step. Deposition of Ti thin film on top of these two types of substrate using DC magnetron sputtering method also showed better adhesion of Ti film on glass for the second type of cleaning method. Scanning electron microscopy (SEM) analyses of the lap shear tested failed surfaces for these two kinds of samples revealed strong bond for samples prepared by second cleaning method compared to first cleaning method. Characterization of these two sets of samples using X-ray photoelectron spectroscopy (XPS) has shown excellent contamination removal for the second cleaning method. This modification is believed to be due to reduction of carbon contamination. 相似文献
9.
Shaheen Faizi Humaira Siddiqi Aneela Naz Samina Bano Lubna 《Helvetica chimica acta》2010,93(3):466-481
An H/D exchange process in patuletin ( 1 ) and its derivatives in D‐donor solvents (e.g., CF3COOD), which occurs regioselectively at C(8) was observed for the first time during NMR studies. The effect of substituents and temperature on the deuteration of various flavonoids (see Fig. 1) which include apigenin, chrysin, galangin, kaempferol, luteolin, morin, myricetin, patuletin, patulitrin, and quercetin, as well as derivatives of patuletin was examined extensively under NMR conditions. The rate constant of deuteration at C(8) of patuletin ( 1 ) and two flavones, luteolin ( 3 ) and apigenin ( 12 ), was also determined in CF3COOD. The D‐atom was introduced into the flavonoids via a keto–enol tautomerism (Scheme 1). During these studies, monodeuterated patuletin was also obtained as a new compound. The examined flavonoids have been reported to possess significant pharmacological activities, and their deuterated derivatives would be of importance for the identification and quantification of these compounds in biological matrices. 相似文献
10.
Hanieh Ghadimi Ramin M.A.TehraniAbdussalam Salhin Mohamed Ali Norita MohamedSulaiman Ab Ghani 《Analytica chimica acta》2013
A novel glassy carbon electrode (GCE) modified with a composite film of poly (4-vinylpyridine) (P4VP) and multiwalled carbon nanotubes (P4VP/MWCNT GCE) was used for the voltammetric determination of paracetamol (PCT). This novel electrode displayed a combined effect of P4VP and MWCNT on the electro-oxidation of PCT in a solution of phosphate buffer at pH 7. Hence, conducting properties of P4VP along with the remarkable physical properties of MWCNTs might have combined effects in enhancing the kinetics of PCT oxidation. The P4VP/MWCNT GCE has also demonstrated excellent electrochemical activity toward PCT oxidation compared to that with bare GCE and MWCNT GCE. The anodic peak currents of PCT on the P4VP/MWCNT GCE were about 300 fold higher than that of the non-modified electrodes. By applying differential pulse voltammetry technique under optimized experimental conditions, a good linear ratio of oxidation peak currents and concentrations of PCT over the range of 0.02–450 μM with a limit of detection of 1.69 nM were achieved. This novel electrode was stable for more than 60 days and reproducible responses were obtained at 99% of the initial current of PCT without any influence of physiologically common interferences such as ascorbic acid and uric acid. The application of this electrode to determine PCT in tablets and urine samples was proposed. 相似文献