排序方式: 共有5条查询结果,搜索用时 31 毫秒
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Eva C. Sonnenschein Marc Stierhof Stephan Goralczyk Floriane M. Vabre Leonie Pellissier Kine Østnes Hanssen Mercedes de la Cruz Caridad Díaz Peter de Witte Daniëlle Copmans Jeanette Hammer Andersen Espen Hansen Venke Kristoffersen José R. Tormo Rainer Ebel Bruce F. Milne Hai Deng Lone Gram Jioji N. Tabudravu 《Tetrahedron》2017,73(18):2633-2637
A new siderophore containing a 4,5-dihydroimidazole moiety was isolated from Pseudoalteromonas piscicida S2040 together with myxochelins A and B, alteramide A and its cycloaddition product, and bromo- and dibromoalterochromides. The structure of pseudochelin A was established by spectroscopic techniques including 2D NMR and MS/MS fragmentation data. In bioassays selected fractions of the crude extract of S2040 inhibited the opportunistic pathogen Pseudomonas aeruginosa. Pseudochelin A displayed siderophore activity in the chrome azurol S assay at concentrations higher than 50 μM, and showed weak activity against the fungus Aspergillus fumigatus, but did not display antibacterial, anti-inflammatory or anticonvulsant activity. 相似文献
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Venke Kristoffersen Marte Jenssen Heba Raid Jawad Johan Isaksson Espen H. Hansen Teppo Rm Kine
. Hansen Jeanette Hammer Andersen 《Molecules (Basel, Switzerland)》2021,26(17)
The Lacinutrix genus was discovered in 2005 and includes 12 Gram-negative bacterial species. To the best of our knowledge, the secondary metabolite production potential of this genus has not been explored before, and examination of Lacinutrix species may reveal novel chemistry. As part of a screening project of Arctic marine bacteria, the Lacinutrix sp. strain M09B143 was cultivated, extracted, fractionated and tested for antibacterial and cytotoxic activities. One fraction had antibacterial activity and was subjected to mass spectrometry analysis, which revealed two compounds with elemental composition that did not match any known compounds in databases. This resulted in the identification and isolation of two novel isobranched lyso-ornithine lipids, whose structures were elucidated by mass spectrometry and NMR spectroscopy. Lyso-ornithine lipids consist of a 3-hydroxy fatty acid linked to the alpha amino group of an ornithine amino acid through an amide bond. The fatty acid chains were determined to be iso-C15:0 (1) and iso-C16:0 (2). Compound 1 was active against the Gram-positive S. agalactiae, while 2 showed cytotoxic activity against A2058 human melanoma cells. 相似文献
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Marte Jenssen Venke Kristoffersen Kumar Motiram-Corral Johan Isaksson Teppo Rm Jeanette H. Andersen Espen H. Hansen Kine
stnes Hansen 《Molecules (Basel, Switzerland)》2021,26(24)
As part of our search for bioactive metabolites from understudied marine microorganisms, the new chlorinated metabolite chlovalicin B (1) was isolated from liquid cultures of the marine basidiomycete Digitatispora marina, which was collected and isolated from driftwood found at Vannøya, Norway. The structure of the novel compound was elucidated by spectroscopic methods including 1D and 2D NMR and analysis of HRMS data, revealing that 1 shares its molecular scaffold with a previously isolated compound, chlovalicin. This represents the first compound isolated from the Digitatispora genus, and the first reported fumagillin/ovalicin-like compound isolated from Basidiomycota. Compound 1 was evaluated for antibacterial activities against a panel of five bacteria, its ability to inhibit bacterial biofilm formation, for antifungal activity against Candida albicans, and for cytotoxic activities against malignant and non-malignant human cell lines. Compound 1 displayed weak cytotoxic activity against the human melanoma cell line A2058 (~50% survival at 50 µM). No activity was detected against biofilm formation or C. albicans at 50 µM, or against bacterial growth at 100 µM nor against the production of cytokines by the human acute monocytic leukemia cell line THP-1 at 50 µM. 相似文献
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Direct determination of serotonin (5-HT) in gut lavage fluid from patients examined due to various gastrointestinal complaints has been achieved. The method involves addition of 5-methoxytryptamine (5-CH3O-HT) internal standard, centrifugation, filtration and injection of the sample supernatant in a liquid chromatographic system coupled to an ion trap tandem mass detector. Electro-spray in positive mode was used to isolate and fragment the protonated ions [5-HT+H]+ and [5-CH3O-HT+H]+ signals 177 and 191 m/z, respectively. Quantification was carried out by extracting the ion fragment chromatograms at 160 and 174 m/z for 5-HT and 5-CH3O-HT, respectively. The relationship 5-HT/5-CH3O-HT was modelled by using a simultaneous design in order to estimate the optimal amount of internal standard to be added to the samples prior to quantification. 相似文献
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