X-ray emission from clusters of galaxies and cosmological parameters

Clusters of galaxies are excellent probes of cosmic structure and evolution. X-ray studies of clusters provide some of their key parameters, viz., temperature of the hot intra-cluster gas, its metallicity, X-ray luminosity and surface brightness giving mass distribution and mass-flow rate in the case of cooling flows. X-ray measurements for a large sample of clusters have lead to estimates of the total gravitating mass in them, which can be compared to the virial masses derived from dynamical considerations and gravitational lensing in some of them. X-ray derived total masses are consistent with masses obtained from the other methods after the effects due to the presence of cooling flows are taken into account in the analyses. Estimated virial masses, lack of evolution in X-ray properties, and detection of several very hot clusters at high redshifts indicate a Universe with a low value (≤ 0.3) for the Ω parameter.     

Exotic decay in cerium isotopes

Half life for the emission of exotic clusters like ⁸Be, ¹²C, ¹⁶O, ²⁰Ne, ²⁴Mg and ²⁸Si are computed taking Coulomb and proximity potentials as interacting barrier and many of these are found well within the present upper limit of measurement. These results lie very close to those values reported by Shanmugam et al using their cubic plus Yukawa plus exponential model (CYEM). It is found that ¹²C and ¹⁶O emissions from ¹¹⁶Ce and ¹⁶O from ¹¹⁸Ce are most favorable for measurement (T _1/2<10¹⁰ s). Lowest half life time for ¹⁶O emission from ¹¹⁶Ce stress the role of doubly magic ¹⁰⁰Sn daughter in exotic decay. Geiger-Nuttall plots were studied for different clusters and are found to be linear. Inclusion of proximity potential will not produce much deviation to linear nature of Geiger-Nuttall plots. It is observed that neutron excess in the parent nuclei slow down the exotic decay process. These findings support the earlier observations of Gupta and collaborators using their preformed cluster model (PCM).     

Protein extraction for proteome analysis from cacao leaves and meristems, organs infected by Moniliophthora perniciosa, the causal agent of the witches' broom disease

Preparation of high-quality proteins from cacao vegetative organs is difficult due to very high endogenous levels of polysaccharides and polyphenols. In order to establish a routine procedure for the application of proteomic and biochemical analysis to cacao tissues, three new protocols were developed; one for apoplastic washing fluid (AWF) extraction, and two for protein extraction--under denaturing and nondenaturing conditions. The first described method allows a quick and easy collection of AWF--using infiltration-centrifugation procedure--that is representative of its composition in intact leaves according to the smaller symplastic contamination detected by the use of the hexose phosphate isomerase marker. Protein extraction under denaturing conditions for 2-DE was remarkably improved by the combination of chemically and physically modified processes including phenol, SDS dense buffer and sonication steps. With this protocol, high-quality proteins from cacao leaves and meristems were isolated, and for the first time well-resolved 1-DE and 2-DE protein patterns of cacao vegetative organs are shown. It also appears that sonication associated with polysaccharide precipitation using tert-butanol was a crucial step for the nondenaturing protein extraction and subsequent enzymatic activity detection. It is expected that the protocols described here could help to develop high-level proteomic and biochemical studies in cacao also being applicable to other recalcitrant plant tissues.     

Preparation and characterization of MgB2 superconductor

The MgB₂ superconductor, synthesized using solid-state and liquid-phase sintering methods, have been characterized for various properties. The upper critical field, irreversibility line and critical current density have been determined using magnetization data. The current-voltage characteristics recorded under an applied magnetic field revealed the existence of vortex glass transition. The surface analysis using X-ray photoelectron spectroscopy shows that MgB₂ is sensitive to atmospheric degradation.