排序方式: 共有21条查询结果,搜索用时 234 毫秒
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G. D. Elsdon J. R. Stubbs E. J. Bartholomew E. C. Raby T. Inouye D. N. Chatterji K. R. Ganguly M. Z. Faruqi L. van Itallie K. Plum D. C. Garratt R. Gager R. M. Mayer R. Berg St. Laguna H. Schönlebe F. Timm L. Servantie F. H. Goldman W. Neugebauer G. Jungmichel H. Schückle L. Pilati und W. Burkhardt 《Fresenius' Journal of Analytical Chemistry》1939,117(7-8):297-304
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Walczak W Pipalia NH Soni M Faruqi AF Ralph H Maxfield FR Webb BL 《Combinatorial chemistry & high throughput screening》2006,9(9):711-718
The conversion of the genomic information produced by the recent sequencing projects into a comprehensive understanding of the human proteome has yet to occur. A new technology that represents a potential bridge between genomics and proteomics is reverse transfection. Reverse transfection cell microarrays are produced by overlaying cDNA arrays with mammalian cells, generating localized clusters of transfected cells with each cluster overexpressing a unique protein. This miniaturized cell-based microarray format affords parallel functional analysis of thousands of cDNA constructs in a high throughput format. In this report we document the development of a co-transfection methodology for reverse transfection applications. The demonstrated high co-transfection efficiency with a "marker" plasmid encoding for GFP enables the identification of transfected cells and eliminates the need for epitope-tagged constructs in cell-based high throughput screening applications using reverse transfection. This co-transfection method was used to study in parallel the structure/function of multiple versions of the v-Src protein using automated fluorescence microscopy. The wild-type v-Src protein and four mutants having insertions or deletions in the SH2 or SH3 domains displayed high levels of tyrosine kinase activity in HEK293T cells. Three other mutated v-Src proteins, including a kinase-dead version, were shown to be defective for tyrosine kinase activity. This reverse co-transfection approach is applicable for high throughput screening of both cDNA libraries and positional scanning recombinant protein libraries. 相似文献
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G. D. Elsdon J. R. Stubbs E. J. Bartholomew E. C. Raby T. Inouye D. N. Chatterji K. R. Ganguly M. Z. Faruqi L. van Itallie K. Plum D. C. Garratt R. Gager R. M. Mayer R. Berg St. Laguna H. Schönlebe F. Timm L. Servantie F. H. Goldman W. Neugebauer G. Jungmichel H. Schückle L. Pilati W. Burkhardt 《Analytical and bioanalytical chemistry》1939,117(7-8):297-304
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Márcio AF Belo-Filho Franklina MB Toledo Bernardo Almada-Lobo 《The Journal of the Operational Research Society》2014,65(11):1735-1747
Setup operations are significant in some production environments. It is mandatory that their production plans consider some features, as setup state conservation across periods through setup carryover and crossover. The modelling of setup crossover allows more flexible decisions and is essential for problems with long setup times. This paper proposes two models for the capacitated lot-sizing problem with backlogging and setup carryover and crossover. The first is in line with other models from the literature, whereas the second considers a disaggregated setup variable, which tracks the starting and completion times of the setup operation. This innovative approach permits a more compact formulation. Computational results show that the proposed models have outperformed other state-of-the-art formulation. 相似文献
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Stability and convergence of the spectral Lagrange-Galerkin method for mixed periodic/non-periodic convection-dominated diffusion problems 总被引:1,自引:0,他引:1
We present a convergence analysis of the spectral Lagrange-Galerkinmethod for mixed periodic/non-periodic convection-diffusionproblems. The scheme is unconditionally stable, independentof the diffusion coefficient, even in the case when numericalquadrature is used. The theoretical predictions are illustratedby a series of numerical experiments. For the periodic case,our results present a significant improvement on those givenby Süli & Ware (1991) SIAM J. Numer.Anal.28, 423-445). 相似文献
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Kanti Jotania Padminin Yadav S. A. Faruqi 《International Journal of Theoretical Physics》2011,50(5):1424-1443
The present study deals with a spatially homogeneous and anisotropic Bianchi-II cosmological models representing massive strings
by applying the variation law for generalized Hubble’s parameter that yields a constant value of deceleration parameter. We
find that the constant value of deceleration parameter is reasonable for the present day universe. The variation law for Hubble’s
parameter generates two types of solutions for the average scale factor, one is of power-law type and other is of the exponential
form. Using these two forms, Einstein’s field equations are solved separately that correspond to expanding singular and non-singular
models of the universe respectively. The energy-momentum tensor for such string as formulated by Letelier (Phys. Rev. D 28:2414,
1983) is used to construct massive string cosmological models for which we assume that the expansion (θ) in the model is proportional to the component s11\sigma^{1}_{1} of the shear tensor sji\sigma^{j}_{i}. This condition leads to A=(BC)
m
, where A, B and C are the metric coefficients and m is proportionality constant. Our models are in accelerating phase which is consistent to the recent observations. The cosmological
constant Λ is found to be a decreasing function of time and it approaches a small positive value at present epoch which is
in good agreement by the results from recent supernovae observations. Some physical and geometric behaviour of the models
are also discussed. 相似文献